Comparative Pharmacokinetic Studies of Four Ginsenosides in Rat Plasma by UPLC-MSMS after Oral Administration of Panax quinquefolius-Acorus gramineus and Panax quinquefolius Extracts
Joint Authors
Yan, Xinjia
Xie, Hailong
Wang, Dongxue
Zhang, Wenjun
Zhao, Ying
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-11-06
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Panax quinquefolius (PQ) and Acorus gramineus (AG) are drug target pairs in traditional Chinese medicine (TCM), which are used to treat age-related diseases.
In the present study, we simultaneously determined the contents of four main bioactive ginsenosides (Rb1, Rb2, Rd, and Re) in rat plasma using an ultrahigh-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method.
Plasma specimens were purified by using the solid-phase extraction procedure, and separation was performed on Waters ACQUITY UPLC BEH C18 (100 mm × 2.1 mm, 1.7 μm) in multiple reaction monitoring (MRM) mode and negative electrospray ionization (ESI) mode.
The established UPLC-MS/MS method showed good linear correlation (r ≥ 0.9978), stability (−11.93 to 12.11%), precision (RSD < 14.63%), and recovery (76.43%–95.20%).
The lower limit of quantification was 3.6 ng/mL for Rb1, 1.6 ng/mL for Rb2, 1.2 ng/mL for Rd, and 2.5 ng/mL for Re.
This validated method was successfully employed to investigate the pharmacokinetics of the four ginsenosides in rat plasma after oral administration of PQ-AG and PQ extracts.
The results revealed the pharmacokinetic profiles of PQ-AG drug pair and clarified that AG played a critical role in stimulating the absorption of active ginsenosides in PQ.
Collectively, our findings provided valid and reliable evidence for the rational use of PQ-AG in clinical practice.
American Psychological Association (APA)
Xie, Hailong& Wang, Dongxue& Zhang, Wenjun& Yan, Xinjia& Zhao, Ying. 2019. Comparative Pharmacokinetic Studies of Four Ginsenosides in Rat Plasma by UPLC-MSMS after Oral Administration of Panax quinquefolius-Acorus gramineus and Panax quinquefolius Extracts. Journal of Analytical Methods in Chemistry،Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1169170
Modern Language Association (MLA)
Xie, Hailong…[et al.]. Comparative Pharmacokinetic Studies of Four Ginsenosides in Rat Plasma by UPLC-MSMS after Oral Administration of Panax quinquefolius-Acorus gramineus and Panax quinquefolius Extracts. Journal of Analytical Methods in Chemistry No. 2019 (2019), pp.1-13.
https://search.emarefa.net/detail/BIM-1169170
American Medical Association (AMA)
Xie, Hailong& Wang, Dongxue& Zhang, Wenjun& Yan, Xinjia& Zhao, Ying. Comparative Pharmacokinetic Studies of Four Ginsenosides in Rat Plasma by UPLC-MSMS after Oral Administration of Panax quinquefolius-Acorus gramineus and Panax quinquefolius Extracts. Journal of Analytical Methods in Chemistry. 2019. Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1169170
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1169170