Determination of Anlotinib, a Tyrosine Kinase Inhibitor, in Rat Plasma by UHPLC-MSMS and Its Application to a Pharmacokinetic Study
Joint Authors
Wang, Zhe
Huang, Cheng-ke
Lian, Lejing
Dong, Yan-yan
Cui, Xiao
Qian, Jian-chang
Chen, Rui-jie
Sun, Wei
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-12-10
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Anlotinib is a novel inhibitor of receptor kinase tyrosine with multitargets and has a broad spectrum of inhibitory action on tumor angiogenesis and growth.
A simple and rapid UHPLC-MS/MS bioanalytical method was validated for the determination of anlotinib in rat plasma, using imatinib as an internal standard.
An Acquity BEH C18 column was used to separate analytes.
The eluents consisted of formic acid/water (0.1 : 100, v/v) and acetonitrile with a mobile phase.
A triple quadrupole mass spectrometer was operated for the quantification with multiple reaction monitoring (MRM) to determine transitions: 408.2 ⟶ 339.1 for anlotinib, and 494.3 ⟶ 394.1 for imatinib.
The validated range was 0.1–50 ng/mL for anlotinib.
Mean recovery rate of anlotinib in plasma was ≥99.32% and reproducible.
Also, the intra- and interday precisions were both below 15%.
This robust method was successfully applied to support the pharmacokinetic study of anlotinib in rats.
American Psychological Association (APA)
Wang, Zhe& Lian, Lejing& Dong, Yan-yan& Cui, Xiao& Qian, Jian-chang& Huang, Cheng-ke…[et al.]. 2019. Determination of Anlotinib, a Tyrosine Kinase Inhibitor, in Rat Plasma by UHPLC-MSMS and Its Application to a Pharmacokinetic Study. Journal of Analytical Methods in Chemistry،Vol. 2019, no. 2019, pp.1-7.
https://search.emarefa.net/detail/BIM-1169172
Modern Language Association (MLA)
Wang, Zhe…[et al.]. Determination of Anlotinib, a Tyrosine Kinase Inhibitor, in Rat Plasma by UHPLC-MSMS and Its Application to a Pharmacokinetic Study. Journal of Analytical Methods in Chemistry No. 2019 (2019), pp.1-7.
https://search.emarefa.net/detail/BIM-1169172
American Medical Association (AMA)
Wang, Zhe& Lian, Lejing& Dong, Yan-yan& Cui, Xiao& Qian, Jian-chang& Huang, Cheng-ke…[et al.]. Determination of Anlotinib, a Tyrosine Kinase Inhibitor, in Rat Plasma by UHPLC-MSMS and Its Application to a Pharmacokinetic Study. Journal of Analytical Methods in Chemistry. 2019. Vol. 2019, no. 2019, pp.1-7.
https://search.emarefa.net/detail/BIM-1169172
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1169172