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Rapid-Response and Highly Sensitive Boronate Derivative-Based Fluorescence Probe for Detecting H2O2 in Living Cells
Joint Authors
Selvaraj, Muthusamy
Rajalakshmi, Kanagaraj
Nam, Yun-Sik
Lee, Yeonhee
Kim, Byoung Chan
Pai, Sung Jin
Han, Sang Soo
Lee, Kang-Bong
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-05-02
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Intracellular H2O2 monitoring is important and has driven researchers to pursue advancements for the rapid identification of H2O2, since H2O2 is short-lived in cell lines.
An arylboronate derivative has been investigated as a chemospecific fluorescence recognition agent for H2O2.
Triphenylimidazoleoxadiazolephenyl (TPIOP) boronate was contrived as a novel candidate for the rapid and sensitive recognition of H2O2.
The probe was conjugated using the TPIOP functional group acting as an excellent fluorescent enhancer.
The TPIOP group stimulated the polarization of C–B bond due to its extended π-conjugation, which included heteroatoms, and induced the production of rapid signal because of the highly polar C–B bond along with the corresponding boronate unit.
While H2O2 reacts with TPIOP boronate, its nucleophilic addition to the boron generates a charged tetrahedral boronate complex, and then the C–B bond migrates toward one of the electrophilic peroxide oxygen atoms.
The resulting boronate ester is then hydrolyzed by water into a phenol, which significantly enhances fluorescence through aggregation-induced emission.
The TPIOP boronate probe responded to H2O2 rapidly, within 2 min, and exhibited high sensitivity with a limit of detection of 8 nM and a 1000-fold selectivity in the presence of other reactive oxygen species.
Therefore, the developed TPIOP boronate chemodosimeter was successfully utilized to visualize and quantify intracellular H2O2 from human breast cancer (MCF-7) cells, as well as gaseous and aqueous H2O2 from environmental samples using Whatman paper strips coated with TPIOP boronate.
American Psychological Association (APA)
Selvaraj, Muthusamy& Rajalakshmi, Kanagaraj& Nam, Yun-Sik& Lee, Yeonhee& Kim, Byoung Chan& Pai, Sung Jin…[et al.]. 2019. Rapid-Response and Highly Sensitive Boronate Derivative-Based Fluorescence Probe for Detecting H2O2 in Living Cells. Journal of Analytical Methods in Chemistry،Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1169180
Modern Language Association (MLA)
Selvaraj, Muthusamy…[et al.]. Rapid-Response and Highly Sensitive Boronate Derivative-Based Fluorescence Probe for Detecting H2O2 in Living Cells. Journal of Analytical Methods in Chemistry No. 2019 (2019), pp.1-12.
https://search.emarefa.net/detail/BIM-1169180
American Medical Association (AMA)
Selvaraj, Muthusamy& Rajalakshmi, Kanagaraj& Nam, Yun-Sik& Lee, Yeonhee& Kim, Byoung Chan& Pai, Sung Jin…[et al.]. Rapid-Response and Highly Sensitive Boronate Derivative-Based Fluorescence Probe for Detecting H2O2 in Living Cells. Journal of Analytical Methods in Chemistry. 2019. Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1169180
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1169180