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Simultaneous Determination of Five Chromones of Radix Saposhnikoviae Extract in Rat Plasma by UPLC-MSMS: Application to a Comparative Pharmacokinetic Study in Normal and Febrile Rats
Joint Authors
Shan, Guoshun
Gao, Hui
Meng, Li
Chen, Bin
Liu, Peng-peng
Zhang, Fan
Jia, Tian-zhu
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-02-26
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
A rapid and sensitive quantitative analytical method was established for the simultaneous determination of five chromones (prim-O-glucosylcimifugin, cimifugin, 4′-O-β-D-glucosyl-5-O-methylvisamminol, 5-O-methylvisammiol, and sec-o-glucosylhamaudol) in the plasma of RS-treated rats for the first time using ultra performance liquid chromatography- (UPLC-) tandem mass spectrometry.
The Waters Acquity UPLC BEH C18 (50 mm × 2.1 mm, 1.7 μm) was used as the chromatographic column, 0.1% formic acid water and 0.1% formic acid acetonitrile comprised the mobile phases, and all samples were determined under positive ion mode.
The results showed that all analytes had good linearity (r>0.9902), between-day and within-day precisions less than 15%, accuracy between −5.50% and 5.53%, and extraction recovery between 88.26% and 97.65%.
Both the matrix effect and stability met the requirements.
This method was successfully applied for the comparative pharmacokinetics of five active components of RS in normal and febrile rats.
The results showed that the pharmacokinetic behavior of RS extract significantly differed between the two types of rats.
American Psychological Association (APA)
Meng, Li& Gao, Hui& Chen, Bin& Liu, Peng-peng& Shan, Guoshun& Zhang, Fan…[et al.]. 2019. Simultaneous Determination of Five Chromones of Radix Saposhnikoviae Extract in Rat Plasma by UPLC-MSMS: Application to a Comparative Pharmacokinetic Study in Normal and Febrile Rats. Journal of Analytical Methods in Chemistry،Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1169229
Modern Language Association (MLA)
Meng, Li…[et al.]. Simultaneous Determination of Five Chromones of Radix Saposhnikoviae Extract in Rat Plasma by UPLC-MSMS: Application to a Comparative Pharmacokinetic Study in Normal and Febrile Rats. Journal of Analytical Methods in Chemistry No. 2019 (2019), pp.1-11.
https://search.emarefa.net/detail/BIM-1169229
American Medical Association (AMA)
Meng, Li& Gao, Hui& Chen, Bin& Liu, Peng-peng& Shan, Guoshun& Zhang, Fan…[et al.]. Simultaneous Determination of Five Chromones of Radix Saposhnikoviae Extract in Rat Plasma by UPLC-MSMS: Application to a Comparative Pharmacokinetic Study in Normal and Febrile Rats. Journal of Analytical Methods in Chemistry. 2019. Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1169229
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1169229