A Simple, Rapid, Fluorometric Assay for Dopamine by In Situ Reaction of Boronic Acids and cis-Diol
Joint Authors
Liu, Xiaoxia
Tian, Miaomiao
Gao, Wenmei
Zhao, Jinzhong
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-03-27
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
An efficient, sensitive, and low-cost method has been developed for turn-on fluorescence sensing of dopamine (DA).
The method relies on the rapid reaction of DA and 3-Hydroxyphenylboronic acid (3-HPBA) via specific recognition between boronic acids and cis-diol of DA in alkaline solution.
The reaction product shows an excitation wavelength of 417 nm and the maximum emission peak at 470 nm.
The proposed method allows the determination of DA in the range of 50 nM–25 μM, and the whole detection can be completed within 5 minutes.
Furthermore, the presented approach has good selectivity and has been successfully applied to DA sensing in human serum samples, showing great potential in clinical diagnosis.
American Psychological Association (APA)
Liu, Xiaoxia& Tian, Miaomiao& Gao, Wenmei& Zhao, Jinzhong. 2019. A Simple, Rapid, Fluorometric Assay for Dopamine by In Situ Reaction of Boronic Acids and cis-Diol. Journal of Analytical Methods in Chemistry،Vol. 2019, no. 2019, pp.1-7.
https://search.emarefa.net/detail/BIM-1169233
Modern Language Association (MLA)
Liu, Xiaoxia…[et al.]. A Simple, Rapid, Fluorometric Assay for Dopamine by In Situ Reaction of Boronic Acids and cis-Diol. Journal of Analytical Methods in Chemistry No. 2019 (2019), pp.1-7.
https://search.emarefa.net/detail/BIM-1169233
American Medical Association (AMA)
Liu, Xiaoxia& Tian, Miaomiao& Gao, Wenmei& Zhao, Jinzhong. A Simple, Rapid, Fluorometric Assay for Dopamine by In Situ Reaction of Boronic Acids and cis-Diol. Journal of Analytical Methods in Chemistry. 2019. Vol. 2019, no. 2019, pp.1-7.
https://search.emarefa.net/detail/BIM-1169233
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1169233