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Comparison of Phenolic Compound Separations by HPTLC and PPEC with SDS as the Mobile Phase Component
Joint Authors
Polak, Beata
Traczuk, Adam
Kamińska, Marta
Kozyra, Małgorzata
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-01-10
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
The application of the surfactant (sodium dodecyl sulphate, SDS) as the component of the water-organic mobile phase in thin-layer chromatography and pressurized planar electrochromatography is presented.
The influence of various variables on the separation of various phenolic compounds (flavonoids and phenolic acids) as model compounds with systems containing surfactant is discussed.
The effect of concentration of butanol and SDS as well as pH of the mobile phase buffer on migration distance of the solute zones is investigated.
The presence of SDS in the eluent affects the butanol solubility in the mobile phase.
It allows using higher organic solvent concentration systems compared with the mode without surfactant.
The amount of SDS in the eluent has the effect on the solute retention, whereas the eluent buffer pH affects the migration distances of ionisable phenolic acids both in HPTLC and PPEC.
The migration distances of flavonoid glycosides are considerably longer than those of pure flavonoids.
Considering second group of investigated solutes, derivatives of the benzoic acid migrate longer distances in comparison with the cinnamic acid ones.
In addition, in the majority of experiments, ionisable compounds (phenolic acids) migrate longer distances in PPEC than nonionisable compounds (flavonoids).
Additionally, the order of solutes differs in the PPEC and HPTLC system.
American Psychological Association (APA)
Polak, Beata& Traczuk, Adam& Kamińska, Marta& Kozyra, Małgorzata. 2019. Comparison of Phenolic Compound Separations by HPTLC and PPEC with SDS as the Mobile Phase Component. Journal of Analytical Methods in Chemistry،Vol. 2019, no. 2019, pp.1-15.
https://search.emarefa.net/detail/BIM-1169243
Modern Language Association (MLA)
Polak, Beata…[et al.]. Comparison of Phenolic Compound Separations by HPTLC and PPEC with SDS as the Mobile Phase Component. Journal of Analytical Methods in Chemistry No. 2019 (2019), pp.1-15.
https://search.emarefa.net/detail/BIM-1169243
American Medical Association (AMA)
Polak, Beata& Traczuk, Adam& Kamińska, Marta& Kozyra, Małgorzata. Comparison of Phenolic Compound Separations by HPTLC and PPEC with SDS as the Mobile Phase Component. Journal of Analytical Methods in Chemistry. 2019. Vol. 2019, no. 2019, pp.1-15.
https://search.emarefa.net/detail/BIM-1169243
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1169243