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The Application and Analytical Pathway of Dexmedetomidine in IschemiaReperfusion Injury
Joint Authors
Tang, Ying
Jia, Changxin
He, Jianshuai
Zhao, Yang
Chen, Huayong
Wang, Shilei
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-12-27
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Ischemia/reperfusion cerebral injury can cause serious damage to nerve cells.
The injured organelles are cleared by autophagy eventually, which is critical for cell survival.
Dexmedetomidine is neuroprotective in various ischemia/reperfusion models.
Mitochondrial calcium uniporter (MCU) is the most important channel of mitochondrial Ca2+ influx into mitochondria, where Ca2+ has a potential effect on mitochondrial autophagy.
However, the role of MCU in the changes of mitophagy and autophagy caused by dexmedetomidine is unknown.
In this study, we constructed an in vitro I/R model by subjecting the oxygen and glucose deprivation/reperfusion model to SH-SY5Y cells to mimic the cerebral I/R injury.
We found that postconditioning with dexmedetomidine and 3-methyladenine (3MA, an autophagy inhibitor) increased the cell survival meanwhile reduced the production of autophagic vesicles and the expression of LC3 and Beclin 1.
This process also increased the expression of BCL-2, P62, and TOM20.
After applied with spermine (MCU-specific agonist), the expression of autophagy proteins by dexmedetomidine was reversed, and the same changes were also observed in immunofluorescence.
The results of our study suggested that dexmedetomidine can inhibit MCU and reduce excessive mitophagy and autophagy for conferring protection against I/R injury.
American Psychological Association (APA)
Tang, Ying& Jia, Changxin& He, Jianshuai& Zhao, Yang& Chen, Huayong& Wang, Shilei. 2019. The Application and Analytical Pathway of Dexmedetomidine in IschemiaReperfusion Injury. Journal of Analytical Methods in Chemistry،Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1169253
Modern Language Association (MLA)
Tang, Ying…[et al.]. The Application and Analytical Pathway of Dexmedetomidine in IschemiaReperfusion Injury. Journal of Analytical Methods in Chemistry No. 2019 (2019), pp.1-10.
https://search.emarefa.net/detail/BIM-1169253
American Medical Association (AMA)
Tang, Ying& Jia, Changxin& He, Jianshuai& Zhao, Yang& Chen, Huayong& Wang, Shilei. The Application and Analytical Pathway of Dexmedetomidine in IschemiaReperfusion Injury. Journal of Analytical Methods in Chemistry. 2019. Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1169253
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1169253