Pharmacological Evaluation of Secondary Metabolites and Their Simultaneous Determination in the Arabian Medicinal Plant Plicosepalus curviflorus Using HPTLC Validated Method
Joint Authors
Khan, Shabana
Perveen, Shagufta
Siddiqui, Nasir A.
Al-Taweel, Areej Mohammad
Orfali, Raha
Alam, Perwez
Alhowiriny, Tawfeq Abdullah
Al-Yahya, Sami
Majrashi, Najwa Mohammed
Ameen, Fuad
Alluhayb, Khulud
Alghanem, Bandar
Shaibah, Hayat
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-03-19
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Plicosepalus is an important genus of the Loranthaceae family, and it is a semiparasitic plant grown in Saudi Arabia, traditionally used as a cure for diabetes and cancer in human and for increasing lactation in cattle.
A flavonoid quercetin (P1), (-)-catechin (P2), and a flavane gallate 2S,3R-3,3′,4′,5,7-pentahydroxyflavane-5-O-gallate (P3) were isolated from the methanol extract of the aerial parts of P.
curviflorus (PCME).
The PCME and the isolated compounds were subjected to pharmacological assays to estimate peroxisome proliferator-activated receptors PPARα and PPARγ agonistic, anti-inflammatory, cytotoxic, and antimicrobial activities.
Results proved for the first time the dual PPAR activation effect of the PCME and catechin (P2), in addition to the promising anti-inflammatory activity of the flavonoid quercetin (P1).
Interestingly, both PCME and isolated compounds showed potent antioxidant activities while no antimicrobial effect against certain microbial strains had been reported from the extract and the isolated compounds.
Based on the pharmacological importance of these compounds, an HPTLC validated method was developed for the simultaneous estimation of these compounds in PCME.
It was found to furnish a compact and sharp band of compounds P1, P2, and P3 at Rf = 0.34, 0.47, and 0.65, respectively, using dichloromethane, methanol, and formic acid (90 : 9.5 : 0.5, (v/v/v)) as the mobile phase.
Compounds P1, P2, and P3 were found to be 11.06, 10.9, 6.96 μg/mg, respectively, in PCME.
The proposed HPTLC method offers a sensitive, precise, and specific analytical tool for the quantification of quercetin, catechin, and flavane gallates in P.
curviflorus.
American Psychological Association (APA)
Orfali, Raha& Perveen, Shagufta& Siddiqui, Nasir A.& Alam, Perwez& Alhowiriny, Tawfeq Abdullah& Al-Taweel, Areej Mohammad…[et al.]. 2019. Pharmacological Evaluation of Secondary Metabolites and Their Simultaneous Determination in the Arabian Medicinal Plant Plicosepalus curviflorus Using HPTLC Validated Method. Journal of Analytical Methods in Chemistry،Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1169261
Modern Language Association (MLA)
Orfali, Raha…[et al.]. Pharmacological Evaluation of Secondary Metabolites and Their Simultaneous Determination in the Arabian Medicinal Plant Plicosepalus curviflorus Using HPTLC Validated Method. Journal of Analytical Methods in Chemistry No. 2019 (2019), pp.1-8.
https://search.emarefa.net/detail/BIM-1169261
American Medical Association (AMA)
Orfali, Raha& Perveen, Shagufta& Siddiqui, Nasir A.& Alam, Perwez& Alhowiriny, Tawfeq Abdullah& Al-Taweel, Areej Mohammad…[et al.]. Pharmacological Evaluation of Secondary Metabolites and Their Simultaneous Determination in the Arabian Medicinal Plant Plicosepalus curviflorus Using HPTLC Validated Method. Journal of Analytical Methods in Chemistry. 2019. Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1169261
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1169261