Detection of OXA-23 among carbapenem resistant clinical isolates of klebsiella pneumoniae in Hilla

Abstract EN

This study aimed to assess the presence of blaOXA-23 in clinical isolates of Klebsiella pneumoniae.

During the period from April to August 2011, a total of 801 various clinical samples were collected from different hospitals in Hilla city.

One hundred and seventy seven (22%) isolates were diagnosed as Klebsiella spp.,117 isolates belonged to K.pneumoniae .

High prevalence of K.pneumoniae was detected in Babylon Teaching Hospital for Maternity and Pediatric with 65 isolates.

Regarding clinical samples, highest rate were observed for stool samples 38 (27%) followed by sputum 19 (15%).

All 117 K.pneumoniae isolates were primarily screened for - lactams resistance, 91 (78%) showed positive results for beta lactams .

β- lactam resistance isolates were underwent antimicrobial susceptibility to 26 antibiotics by Kirby-Bauer disk diffusion methods.

High resistance rate was recorded for penicillins (carbenicillin and ampicillin) 99% and 94.5%,respectively .Carbapenem resistance was reported in 17 (18.7%) of K.

pneumoniae isolates.

The presence of blaOXA-23 gene was checked by Polymerase Chain reaction (PCR) and confirmed in 15 (88.2%) of isolates.