Antiaging Factor Klotho Retards the Progress of Intervertebral Disc Degeneration through the Toll-Like Receptor 4-NF-κB Pathway

Joint Authors

Liu, Wenbo
Bi, Fangfang
Wu, Zongtao
Ji, Chen
Chang, Cuicui

Source

International Journal of Cell Biology

Issue

Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-11, 11 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2020-03-19

Country of Publication

Egypt

No. of Pages

11

Main Subjects

Biology

Abstract EN

Antiaging protein Klotho exhibits impressive properties of anti-inflammation, however is declined early after intervertebral disc injury, making Klotho restoration an attractive strategy of treating intervertebral disc inflammatory disorders.

Here, we have found that Klotho is enriched in nucleus pulposus (NP) cells and Klotho overexpression attenuates H2O2-induced acute inflammation essentially via suppressing Toll-like receptor 4 (TLR4).

The proinflammatory NF-κB signaling and cytokine expressions paralleled with Klotho repression and TLR4 elevation in both NP cells (H2O2 treatment) and rat intervertebral disc (needle puncture treatment).

Overexpression of TLR4 downregulated expression of Klotho, whereas interfering TLR4 expression diminished the inhibitory effects of H2O2 on Klotho in NP cells.

Consistently, Klotho knockdown by RNA interferences largely diminished the anti-inflammatory and intervertebral disc protective effects in an Intervertebral Disc Degeneration (IDD) model.

Thus, our study indicates that TLR4-NF-κB signaling and Klotho form a negative-feedback loop in NP cells.

Also, we demonstrate that the expression of Klotho is regulated by the balance between upregulation and downregulation of TLR4-NF-κB signaling.

American Psychological Association (APA)

Bi, Fangfang& Liu, Wenbo& Wu, Zongtao& Ji, Chen& Chang, Cuicui. 2020. Antiaging Factor Klotho Retards the Progress of Intervertebral Disc Degeneration through the Toll-Like Receptor 4-NF-κB Pathway. International Journal of Cell Biology،Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1169274

Modern Language Association (MLA)

Bi, Fangfang…[et al.]. Antiaging Factor Klotho Retards the Progress of Intervertebral Disc Degeneration through the Toll-Like Receptor 4-NF-κB Pathway. International Journal of Cell Biology No. 2020 (2020), pp.1-11.
https://search.emarefa.net/detail/BIM-1169274

American Medical Association (AMA)

Bi, Fangfang& Liu, Wenbo& Wu, Zongtao& Ji, Chen& Chang, Cuicui. Antiaging Factor Klotho Retards the Progress of Intervertebral Disc Degeneration through the Toll-Like Receptor 4-NF-κB Pathway. International Journal of Cell Biology. 2020. Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1169274

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1169274