Determining the Levels of Four Phenylethanoid Glycosides and Five Triterpene Acids in Liuwei Dihuang Capsule Using Solid Phase Extraction with HPLC-UV
Joint Authors
Zhang, Yong
Zou, Xian-Liang
Wang, Yong-Li
Gao, Lu
Chou, Gui-Xin
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-11-07
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
In this study, we used quantitative high-performance liquid chromatography equipped with an ultraviolet detector (HPLC-UV) and solid phase extraction (SPE) to determine the levels of four phenylethanoid glycosides and five triterpene acids in Liuwei Dihuang capsules (LDCs).
LDCs were methanol-extracted and purified using a 500 mg/6 mL silica-based C18 SPE cartridge.
Two elutions were analyzed on a ChromCore C18 column under two HPLC conditions.
To improve the pretreatment clean-up, an array of silica- and polymer-based SPE cartridges were compared.
Both wash and elution steps were also optimized to achieve the highest removal of impurities.
Under optimal chromatographic conditions, good linearity was achieved for all compounds (correlation coefficient of r ≥ 0.999), with a quantification limit ranging from 0.0076 to 0.418 μg/mL.
The method had satisfactory efficiency and reproducibility with recovery rates ranging from 91.6 to 99.3% with a relative standard deviation below 1.5%.
Taken together, this demonstrated SPE as a suitable extension of HPLC-UV for the determination of phenylethanoid glycosides and triterpene acids in complex LDCs.
American Psychological Association (APA)
Zhang, Yong& Zou, Xian-Liang& Wang, Yong-Li& Gao, Lu& Chou, Gui-Xin. 2019. Determining the Levels of Four Phenylethanoid Glycosides and Five Triterpene Acids in Liuwei Dihuang Capsule Using Solid Phase Extraction with HPLC-UV. Journal of Analytical Methods in Chemistry،Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1169285
Modern Language Association (MLA)
Zhang, Yong…[et al.]. Determining the Levels of Four Phenylethanoid Glycosides and Five Triterpene Acids in Liuwei Dihuang Capsule Using Solid Phase Extraction with HPLC-UV. Journal of Analytical Methods in Chemistry No. 2019 (2019), pp.1-9.
https://search.emarefa.net/detail/BIM-1169285
American Medical Association (AMA)
Zhang, Yong& Zou, Xian-Liang& Wang, Yong-Li& Gao, Lu& Chou, Gui-Xin. Determining the Levels of Four Phenylethanoid Glycosides and Five Triterpene Acids in Liuwei Dihuang Capsule Using Solid Phase Extraction with HPLC-UV. Journal of Analytical Methods in Chemistry. 2019. Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1169285
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1169285