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Determination of Morphine in Human Urine by the Novel Competitive Fluorescence Immunoassay
Joint Authors
Cao, Jie
Chen, Xiao-Ying
Zhao, Wu-Rong
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-02-03
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
A competitive fluorescence immunoassay for the identification and quantification of morphine has been developed on the basis of hapten-coated plate format.
Hapten was prepared through covalent conjugating a morphine derivative with albumin bovine.
In the immunoassay, the hapten was inoculated on a 96-well plate and then bound with monoclonal antibodies labeled with a signal indicating dye, fluorescein isothiocyanate (FITC).
Unbound FITC-antibodies were rinsed off from the plate.
The fluorescein intensity decreases in the presence of morphine molecules due to the competitively binding to antibodies against hapten.
The intensity is inversely correlated with the concentration of morphine.
In quantitative analysis for urine samples, we obtained a linearity range of 0.2 μg/mL∼2.5 μg/mL, along with a detection limit of c.a.
1 ng/mL.
The fluorescence immunoassay shows low cross-reactivity (below 10%) to 6-acetylmorphine, 3-acetylmorphine, and heroine.
The developed method produced comparable results to the standard GC-MS/MS method.
In conclusion, a rapid and efficient screening tool for morphine in clinical human urine has been established.
American Psychological Association (APA)
Cao, Jie& Chen, Xiao-Ying& Zhao, Wu-Rong. 2019. Determination of Morphine in Human Urine by the Novel Competitive Fluorescence Immunoassay. Journal of Analytical Methods in Chemistry،Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1169291
Modern Language Association (MLA)
Cao, Jie…[et al.]. Determination of Morphine in Human Urine by the Novel Competitive Fluorescence Immunoassay. Journal of Analytical Methods in Chemistry No. 2019 (2019), pp.1-11.
https://search.emarefa.net/detail/BIM-1169291
American Medical Association (AMA)
Cao, Jie& Chen, Xiao-Ying& Zhao, Wu-Rong. Determination of Morphine in Human Urine by the Novel Competitive Fluorescence Immunoassay. Journal of Analytical Methods in Chemistry. 2019. Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1169291
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1169291