Extracts of Clove (Syzygium aromaticum) Potentiate FMSP-Nanoparticles Induced Cell Death in MCF-7 Cells
Joint Authors
Akhtar, Sultan
Khan, Firdos Alam
Almohazey, Dana
Alomari, Munthar
Almofty, Sarah Ameen
Source
International Journal of Biomaterials
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-08-23
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Both nanoparticles and cloves (Syzygium aromaticum) possess anticancer properties, but they do not elicit a significant response on cancer cells when treated alone.
In the present study, we have tested fluorescent magnetic submicronic polymer nanoparticles (FMSP-nanoparticles) in combination with crude clove extracts on human breast cancer cells (MCF-7) to examine whether the combination approach enhance the cancer cell death.
The MCF-7 cells were treated with different concentrations (1.25 μg/mL, 12.5 μg/mL, 50 μg/mL, 75 μg/mL, and 100 μg/mL) of FMSP-nanoparticles alone and in combination with 50 μg/mL crude clove extracts.
The effects of FMSP-nanoparticles alone and combined with clove extracts were observed after 24 hrs and 48 hrs intervals.
The response of FMSP-nanoparticles-treated cells was evaluated by Trypan Blue, 4′,6-diamidino-2-phenylindole (DAPI), and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays, respectively.
We have demonstrated that cancer cell viability was decreased to 55.40% when treated with FMSP-nanoparticles alone, whereas when cancer cells were treated with FMSP-nanoparticles along with crude clove extracts, the cell viability was drastically decreased to 8.50%.
Both morphological and quantitative data suggest that the combination of FMSP-nanoparticles plus crude clove extracts are more effective in treating cancer cells and we suggest that the combination treatment of nanoparticles along with clove extracts hold a great promise for the cancer treatments.
American Psychological Association (APA)
Khan, Firdos Alam& Akhtar, Sultan& Almohazey, Dana& Alomari, Munthar& Almofty, Sarah Ameen. 2018. Extracts of Clove (Syzygium aromaticum) Potentiate FMSP-Nanoparticles Induced Cell Death in MCF-7 Cells. International Journal of Biomaterials،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1169621
Modern Language Association (MLA)
Khan, Firdos Alam…[et al.]. Extracts of Clove (Syzygium aromaticum) Potentiate FMSP-Nanoparticles Induced Cell Death in MCF-7 Cells. International Journal of Biomaterials No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1169621
American Medical Association (AMA)
Khan, Firdos Alam& Akhtar, Sultan& Almohazey, Dana& Alomari, Munthar& Almofty, Sarah Ameen. Extracts of Clove (Syzygium aromaticum) Potentiate FMSP-Nanoparticles Induced Cell Death in MCF-7 Cells. International Journal of Biomaterials. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1169621
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1169621