The Human IL-23 Decoy Receptor Inhibits T-Cells Producing IL-17 by Genetically Engineered Mesenchymal Stem Cells
Joint Authors
Rostami, Masoumeh
Haidari, Kamran
Shahbazi, Majid
Source
International Journal of Cell Biology
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-12-19
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
The immunomodulatory and self-renewable features of human adipose mesenchymal stem cells (hAD-MSCs) mark their importance in regenerative medicine.
Interleukin 23 (IL- 23) as a proinflammatory cytokine suppresses T regulatory cells (Treg) and promotes the response of T helper 17 (Th17) and T helper 1 (Th1) cells.
This pathway starts inflammation and immunosuppression in several autoimmune diseases.
The current study for producing recombinant IL- 23 decoy receptor (RIL- 23R) using hAD-MSCs as a good candidate for ex vivo cell-based gene therapy purposes reducing inflammation in autoimmune diseases.
hAD-MSCs was isolated from lipoaspirate and then characterized by differentiation.
RIL- 23R was designed and cloned into a pCDH-813A- 1 lentiviral vector.
The transduction of hAD-MSCs was performed at MOI (multiplicity of infection) = 50 with pCDH- EFI α- RIL- 23R- PGK copGFP.
Expressions of RIL- 23R and octamer-binding transcription factor 4 (OCT- 4) were determined by real-time polymerase chain reaction (real time-PCR).
Self-renewing properties were assayed with OCT- 4.
Bioactivity of the designed RIL- 23R was evaluated by IL- 17 and IL- 10 expression of mouse splenocytes.
Cell differentiation confirmed the true isolation of hAD-MSCs from lipoaspirate.
Restriction of the enzyme digestion and sequencing verified the successful cloning of RIL- 23R in the CD813A-1 lentiviral vector.
The green fluorescent protein (GFP) positive transduction rate was up to 90%, and real-time PCR showed the expression level of RIL-23R.
Oct-4 had a similar expression pattern with nontransduced hAD-MSCs and transduced hAD-MSCs/ RIL-23R indicating that lentiviral vector did not affect hAD-MSCs characteristics.
Downregulation of IL-17 and upregulation of IL-10 showed the correct activity of the engineered hAD-MSCs.
The results showed that the transduced hAD-MSCs/ RIL- 23R, expressing IL-23 decoy receptor, can give a useful approach for a basic research on cell-based gene therapy for autoimmune disorders.
American Psychological Association (APA)
Rostami, Masoumeh& Haidari, Kamran& Shahbazi, Majid. 2018. The Human IL-23 Decoy Receptor Inhibits T-Cells Producing IL-17 by Genetically Engineered Mesenchymal Stem Cells. International Journal of Cell Biology،Vol. 2018, no. 2018, pp.1-14.
https://search.emarefa.net/detail/BIM-1170011
Modern Language Association (MLA)
Rostami, Masoumeh…[et al.]. The Human IL-23 Decoy Receptor Inhibits T-Cells Producing IL-17 by Genetically Engineered Mesenchymal Stem Cells. International Journal of Cell Biology No. 2018 (2018), pp.1-14.
https://search.emarefa.net/detail/BIM-1170011
American Medical Association (AMA)
Rostami, Masoumeh& Haidari, Kamran& Shahbazi, Majid. The Human IL-23 Decoy Receptor Inhibits T-Cells Producing IL-17 by Genetically Engineered Mesenchymal Stem Cells. International Journal of Cell Biology. 2018. Vol. 2018, no. 2018, pp.1-14.
https://search.emarefa.net/detail/BIM-1170011
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1170011