Hyperoside Alleviates High Glucose-Induced Proliferation of Mesangial Cells through the Inhibition of the ERKCREBmiRNA-34a Signaling Pathway
Joint Authors
Zhou, Ji-Yin
Zhang, Kebin
Zhang, Le
Dai, Qian
Hu, Lanlan
Yu, Hua
Qiu, Jing
Long, Min
Zhou, Shiwen
Source
International Journal of Endocrinology
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-07-22
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Purpose.
Hyperoside, a flavonoid isolated from conventional medicinal herbs, has been demonstrated to exert a significant protective effect in diabetic nephropathy.
This study aimed to determine the underlying mechanisms, by which hyperoside inhibits high glucose-(HG-) induced proliferation in mouse renal mesangial cells.
Methods.
Mouse glomerular mesangial cells line (SV40-MES13) was used to study the inhibitory effect of hyperoside on cell proliferation induced by 30 mM glucose, which was used to simulate a diabetic condition.
Viable cell count was assessed using the Cell Counting Kit-8 and by the 5-ethynyl-20-deoxyuridine incorporation assay.
The underlying mechanism involving miRNA-34a was further investigated by quantitative RT-PCR and transfection with miRNA-34a agomir.
The phosphorylation levels of extracellular signal-regulated kinases (ERKs) and cAMP-response element-binding protein (CREB) were measured by Western blotting.
The binding region and the critical binding sites of CREB in the miRNA-34a promoter were investigated by the chromatin immunoprecipitation assay and luciferase reporter assay, respectively.
Results.
We found that hyperoside could significantly decrease HG-induced proliferation of SV40-MES13 cells in a dose-dependent manner, without causing obvious cell death.
In addition, hyperoside inhibited the activation of ERK pathway and phosphorylation of its downstream transcriptional factor CREB, as well as the miRNA-34a expression.
We further confirmed that CREB-mediated regulation of miRNA-34a is dependent on the direct binding to specific sites in the promoter region of miRNA-34a.
Conclusion.
Our cumulative results suggested that hyperoside inhibits the proliferation of SV40-MES13 cells through the suppression of the ERK/CREB/miRNA-34a signaling pathway, which provides new insight to the current investigation on therapeutic strategies for diabetic nephropathy.
American Psychological Association (APA)
Zhang, Le& Dai, Qian& Hu, Lanlan& Yu, Hua& Qiu, Jing& Zhou, Ji-Yin…[et al.]. 2020. Hyperoside Alleviates High Glucose-Induced Proliferation of Mesangial Cells through the Inhibition of the ERKCREBmiRNA-34a Signaling Pathway. International Journal of Endocrinology،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1170068
Modern Language Association (MLA)
Zhang, Le…[et al.]. Hyperoside Alleviates High Glucose-Induced Proliferation of Mesangial Cells through the Inhibition of the ERKCREBmiRNA-34a Signaling Pathway. International Journal of Endocrinology No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1170068
American Medical Association (AMA)
Zhang, Le& Dai, Qian& Hu, Lanlan& Yu, Hua& Qiu, Jing& Zhou, Ji-Yin…[et al.]. Hyperoside Alleviates High Glucose-Induced Proliferation of Mesangial Cells through the Inhibition of the ERKCREBmiRNA-34a Signaling Pathway. International Journal of Endocrinology. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1170068
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1170068