SPE-UHPLC-FLD Method for the Simultaneous Determination of Five Anthraquinones in Human Urine Using Mixed-Mode Bis(tetraoxacalix[2]arene[2]triazine) Modified Silica as Sorbent
Joint Authors
Hu, Kai
Qiao, Yonghui
Deng, Zhifen
Wu, Mingxia
Liu, Wei
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-09-28
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The five anthraquinones compounds (including aloe-emodin, emodin, physcion, chrysophanol, and rhein) are regarded as the main effective ingredients in rhubarb (Dahuang in Chinese, one of the commonly used Chinese herbal medicines).
In this work, a simple and effective solid phase extraction (SPE) method based on bis(tetraoxacalix[2]arene[2]triazine) modified silica gel as adsorbent was developed.
Coupled with UHPLC-FLD, the developed method was successfully applied for the measuring of main anthraquinones in human urine after oral administration of the extracts of rhubarb.
To obtain the highest recoveries of the five anthraquinones in the SPE process, the main parameters which may affect extraction efficiency were optimized.
The optimized sorbent amount, sample loading pH, sample loading rate, washing solution, and eluent condition were obtained.
The developed method showed good linearity in 0.012–1.800 μg mL−1 for the five anthraquinones with correlation coefficients more than 0.9993.
The investigated LOD values ranged from 3.9 to 5.7 ng mL−1, while the LOQs were between 12.0 and 18.2 ng mL−1.
The recoveries of the method were also investigated, which were in the range of 94.8–106.6%.
The application of the mixed-mode SPE materials in the proposed method was feasible and simple, and suitable for the enrichment of anthraquinones in urine samples.
American Psychological Association (APA)
Hu, Kai& Qiao, Yonghui& Deng, Zhifen& Wu, Mingxia& Liu, Wei. 2017. SPE-UHPLC-FLD Method for the Simultaneous Determination of Five Anthraquinones in Human Urine Using Mixed-Mode Bis(tetraoxacalix[2]arene[2]triazine) Modified Silica as Sorbent. Journal of Analytical Methods in Chemistry،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1170086
Modern Language Association (MLA)
Hu, Kai…[et al.]. SPE-UHPLC-FLD Method for the Simultaneous Determination of Five Anthraquinones in Human Urine Using Mixed-Mode Bis(tetraoxacalix[2]arene[2]triazine) Modified Silica as Sorbent. Journal of Analytical Methods in Chemistry No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1170086
American Medical Association (AMA)
Hu, Kai& Qiao, Yonghui& Deng, Zhifen& Wu, Mingxia& Liu, Wei. SPE-UHPLC-FLD Method for the Simultaneous Determination of Five Anthraquinones in Human Urine Using Mixed-Mode Bis(tetraoxacalix[2]arene[2]triazine) Modified Silica as Sorbent. Journal of Analytical Methods in Chemistry. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1170086
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1170086