Spectrophotometric Methods for Simultaneous Determination of Oxytetracycline HCl and Flunixin Meglumine in Their Veterinary Pharmaceutical Formulation
Joint Authors
Zaazaa, Hala E.
Abd-Elmonem, Mahmmoud S.
Merey, Hanan A.
Nazlawy, Hagar N.
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-07-24
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Four precise, accurate, selective, and sensitive UV-spectrophotometric methods were developed and validated for the simultaneous determination of a binary mixture of Oxytetracycline HCl (OXY) and Flunixin Meglumine (FLU).
The first method, dual wavelength (DW), depends on measuring the difference in absorbance (ΔA 273.4–327 nm) for the determination of OXY where FLU is zero while FLU is determined at ΔA 251.7–275.7 nm.
The second method, first-derivative spectrophotometric method (1D), depends on measuring the peak amplitude of the first derivative selectively at 377 and 266.7 nm for the determination of OXY and FLU, respectively.
The third method, ratio difference method, depends on the difference in amplitudes of the ratio spectra at ΔP 286.5–324.8 nm and ΔP 249.6–286.3 nm for the determination of OXY and FLU, respectively.
The fourth method, first derivative of ratio spectra method (1DD), depends on measuring the amplitude peak to peak of the first derivative of ratio spectra at 296.7 to 369 nm and 259.1 to 304.7 nm for the determination of OXY and FLU, respectively.
Different factors affecting the applied spectrophotometric methods were studied.
The proposed methods were validated according to ICH guidelines.
Satisfactory results were obtained for determination of both drugs in laboratory prepared mixture and pharmaceutical dosage form.
The developed methods are compared favourably with the official ones.
American Psychological Association (APA)
Merey, Hanan A.& Abd-Elmonem, Mahmmoud S.& Nazlawy, Hagar N.& Zaazaa, Hala E.. 2017. Spectrophotometric Methods for Simultaneous Determination of Oxytetracycline HCl and Flunixin Meglumine in Their Veterinary Pharmaceutical Formulation. Journal of Analytical Methods in Chemistry،Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1170090
Modern Language Association (MLA)
Merey, Hanan A.…[et al.]. Spectrophotometric Methods for Simultaneous Determination of Oxytetracycline HCl and Flunixin Meglumine in Their Veterinary Pharmaceutical Formulation. Journal of Analytical Methods in Chemistry No. 2017 (2017), pp.1-8.
https://search.emarefa.net/detail/BIM-1170090
American Medical Association (AMA)
Merey, Hanan A.& Abd-Elmonem, Mahmmoud S.& Nazlawy, Hagar N.& Zaazaa, Hala E.. Spectrophotometric Methods for Simultaneous Determination of Oxytetracycline HCl and Flunixin Meglumine in Their Veterinary Pharmaceutical Formulation. Journal of Analytical Methods in Chemistry. 2017. Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1170090
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1170090