Validation of an UHPLC-MSMS Method for Screening of Antimicrobial Residues in Eggs and Their Application to Analyses of Eggs from Laying Hens Subjected to Pharmacological Treatment
Joint Authors
Caldeira, Letícia Gomes Magnago
Santos, Flávio Alves
Oliveira, Andréa Melo Garcia de
Lima, Josefa Abucater
Souza, Leonardo Francisco de
Silva, Guilherme Resende da
Assis, Débora Cristina Sampaio de
Cançado, Silvana de Vasconcelos
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-10-23
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
A multiresidue method by UHPLC/MS-MS was optimized and validated for the screening and semiquantitative detection of antimicrobials residues from tetracyclines, aminoglycosides, quinolones, lincosamides, β-lactams, sulfonamides, and macrolides families in eggs.
A qualitative approach was used to ensure adequate sensitivity to detect residues at the level of interest, defined as maximum residue limit (MRL), or less.
The applicability of the methods was assessed by analyzing egg samples from hens that had been subjected to pharmacological treatment with neomycin, enrofloxacin, lincomycin, oxytetracycline, and doxycycline during five days and after discontinuation of medication (10 days).
The method was adequate for screening all studied analytes in eggs, since the performance parameters ensured a false-compliant rate below or equal to 5%, except for flumequine.
In the analyses of eggs from laying hens subjected to pharmacological treatment, all antimicrobial residues were detected throughout the experimental period, even after discontinuation of medication, except for neomycin, demonstrating the applicability of the method for analyses of antimicrobial residues in eggs.
American Psychological Association (APA)
Caldeira, Letícia Gomes Magnago& Santos, Flávio Alves& Oliveira, Andréa Melo Garcia de& Lima, Josefa Abucater& Souza, Leonardo Francisco de& Silva, Guilherme Resende da…[et al.]. 2017. Validation of an UHPLC-MSMS Method for Screening of Antimicrobial Residues in Eggs and Their Application to Analyses of Eggs from Laying Hens Subjected to Pharmacological Treatment. Journal of Analytical Methods in Chemistry،Vol. 2017, no. 2017, pp.1-14.
https://search.emarefa.net/detail/BIM-1170157
Modern Language Association (MLA)
Caldeira, Letícia Gomes Magnago…[et al.]. Validation of an UHPLC-MSMS Method for Screening of Antimicrobial Residues in Eggs and Their Application to Analyses of Eggs from Laying Hens Subjected to Pharmacological Treatment. Journal of Analytical Methods in Chemistry No. 2017 (2017), pp.1-14.
https://search.emarefa.net/detail/BIM-1170157
American Medical Association (AMA)
Caldeira, Letícia Gomes Magnago& Santos, Flávio Alves& Oliveira, Andréa Melo Garcia de& Lima, Josefa Abucater& Souza, Leonardo Francisco de& Silva, Guilherme Resende da…[et al.]. Validation of an UHPLC-MSMS Method for Screening of Antimicrobial Residues in Eggs and Their Application to Analyses of Eggs from Laying Hens Subjected to Pharmacological Treatment. Journal of Analytical Methods in Chemistry. 2017. Vol. 2017, no. 2017, pp.1-14.
https://search.emarefa.net/detail/BIM-1170157
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1170157