Changes of Metabolomic Profile in Helianthus annuus under Exposure to Chromium(VI) Studied by capHPLC-ESI-QTOF-MS and MSMS
Joint Authors
Gonzalez Ibarra, Alan Alexander
Wrobel, Kazimierz
Yanez Barrientos, Eunice
Corrales Escobosa, Alma Rosa
Gutierrez Corona, J. Felix
Enciso Donis, Israel
Wrobel, Katarzyna
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-18, 18 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-11-22
Country of Publication
Egypt
No. of Pages
18
Main Subjects
Abstract EN
The application of capHPLC-ESI-QTOF-MS and MS/MS to study the impact of Cr(VI) on metabolites profile in Helianthus annuus is reported.
Germinated seeds were grown hydroponically in the presence of Cr(VI) (25 mgCr/L) and root extracts of the exposed and control plants were analyzed by untargeted metabolomic approach.
The main goal was to detect which metabolite groups were mostly affected by Cr(VI) stress; two data analysis tools (ProfileAnalysis, Bruker, and online XCMS) were used under criteria of intensity threshold 5 · 104 cps, fold change ≥ 5, and p≤0.01, yielding precursor ions.
Molecular formulas were assigned based on data processing with two computational tools (SIRIUS and MS-Finder); annotation of candidate structures was performed by database search using CSI:FingerID and MS-Finder.
Even though ultimate identification has not been achieved, it was demonstrated that secondary metabolism became activated under Cr(VI) stress.
Among 42 candidate compounds returned from database search for seven molecular formulas, ten structures corresponded to isocoumarin derivatives and eleven were sesquiterpenes or sesquiterpene lactones; three benzofurans and four glycoside or pyrane derivatives of phenolic compounds were also suggested.
To gain further insight on the effect of Cr(VI) in sunflower, isocoumarins and sesquiterpenes were selected as the target compounds for future study.
American Psychological Association (APA)
Gonzalez Ibarra, Alan Alexander& Wrobel, Kazimierz& Yanez Barrientos, Eunice& Corrales Escobosa, Alma Rosa& Gutierrez Corona, J. Felix& Enciso Donis, Israel…[et al.]. 2017. Changes of Metabolomic Profile in Helianthus annuus under Exposure to Chromium(VI) Studied by capHPLC-ESI-QTOF-MS and MSMS. Journal of Analytical Methods in Chemistry،Vol. 2017, no. 2017, pp.1-18.
https://search.emarefa.net/detail/BIM-1170175
Modern Language Association (MLA)
Gonzalez Ibarra, Alan Alexander…[et al.]. Changes of Metabolomic Profile in Helianthus annuus under Exposure to Chromium(VI) Studied by capHPLC-ESI-QTOF-MS and MSMS. Journal of Analytical Methods in Chemistry No. 2017 (2017), pp.1-18.
https://search.emarefa.net/detail/BIM-1170175
American Medical Association (AMA)
Gonzalez Ibarra, Alan Alexander& Wrobel, Kazimierz& Yanez Barrientos, Eunice& Corrales Escobosa, Alma Rosa& Gutierrez Corona, J. Felix& Enciso Donis, Israel…[et al.]. Changes of Metabolomic Profile in Helianthus annuus under Exposure to Chromium(VI) Studied by capHPLC-ESI-QTOF-MS and MSMS. Journal of Analytical Methods in Chemistry. 2017. Vol. 2017, no. 2017, pp.1-18.
https://search.emarefa.net/detail/BIM-1170175
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1170175