Evaluation of Global Genomic DNA Methylation in Human Whole Blood by Capillary Electrophoresis UV Detection
Joint Authors
Mangoni, Arduino A.
Zinellu, Angelo
Paliogiannis, Panagiotis
Pintus, Gianfranco
Sotgia, Salvatore
Sotgiu, Elisabetta
Assaretti, Stefano
Carru, Ciriaco
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-12-03
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Alterations in global DNA methylation are implicated in various pathophysiological processes.
The development of simple and quick, yet robust, methods to assess DNA methylation is required to facilitate its measurement and interpretation in clinical practice.
We describe a highly sensitive and reproducible capillary electrophoresis method with UV detection for the separation and detection of cytosine and methylcytosine, after formic acid hydrolysis of DNA extracted from human whole blood.
Hydrolysed samples were dried and resuspended with water and directly injected into the capillary without sample derivatization procedures.
The use of a run buffer containing 50 mmol/L BIS-TRIS propane (BTP) phosphate buffer at pH 3.25 and 60 mmol/L sodium acetate buffer at pH 3.60 (4 : 1, v/v) allowed full analyte identification within 11 min.
Precision tests indicated an elevated reproducibility with an interassay CV of 1.98% when starting from 2 μg of the extracted DNA.
The method was successfully tested by measuring the DNA methylation degree both in healthy volunteers and in reference calf thymus DNA.
American Psychological Association (APA)
Zinellu, Angelo& Sotgiu, Elisabetta& Assaretti, Stefano& Sotgia, Salvatore& Paliogiannis, Panagiotis& Pintus, Gianfranco…[et al.]. 2017. Evaluation of Global Genomic DNA Methylation in Human Whole Blood by Capillary Electrophoresis UV Detection. Journal of Analytical Methods in Chemistry،Vol. 2017, no. 2017, pp.1-6.
https://search.emarefa.net/detail/BIM-1170198
Modern Language Association (MLA)
Zinellu, Angelo…[et al.]. Evaluation of Global Genomic DNA Methylation in Human Whole Blood by Capillary Electrophoresis UV Detection. Journal of Analytical Methods in Chemistry No. 2017 (2017), pp.1-6.
https://search.emarefa.net/detail/BIM-1170198
American Medical Association (AMA)
Zinellu, Angelo& Sotgiu, Elisabetta& Assaretti, Stefano& Sotgia, Salvatore& Paliogiannis, Panagiotis& Pintus, Gianfranco…[et al.]. Evaluation of Global Genomic DNA Methylation in Human Whole Blood by Capillary Electrophoresis UV Detection. Journal of Analytical Methods in Chemistry. 2017. Vol. 2017, no. 2017, pp.1-6.
https://search.emarefa.net/detail/BIM-1170198
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1170198