One-Step Ultrafiltration Process for Separation and Purification of a Keratinolytic Protease Produced with Feather Meal
Joint Authors
Brandelli, Adriano
Machado, Juliana R.
Severo, Emanuelle E.
de Oliveira, Janaina M. G.
Ores, Joana da C.
Kalil, Susana J.
Source
International Journal of Chemical Engineering
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-06-28
Country of Publication
Egypt
No. of Pages
7
Abstract EN
A purification technique to obtain keratinolytic proteases produced by Bacillus sp.
P45 in a medium containing chicken feather meal as substrate is presented.
The experiments were carried out in a dead-end ultrafiltration unit, and the influence of the membrane cutoff, pH of enzymatic extract, and operating pressure on the purification of keratinase were studied.
The one-step ultrafiltration process with the membrane molecular mass cutoff of 10 kDa at pH 8.0 and operating pressure of 0.147 MPa showed an enzyme recovery of 87.8% and a 4.1-fold purification factor.
It is showed that ultrafiltration could be potentially used in the purification of keratinases.
American Psychological Association (APA)
Machado, Juliana R.& Severo, Emanuelle E.& de Oliveira, Janaina M. G.& Ores, Joana da C.& Brandelli, Adriano& Kalil, Susana J.. 2018. One-Step Ultrafiltration Process for Separation and Purification of a Keratinolytic Protease Produced with Feather Meal. International Journal of Chemical Engineering،Vol. 2018, no. 2018, pp.1-7.
https://search.emarefa.net/detail/BIM-1170205
Modern Language Association (MLA)
Machado, Juliana R.…[et al.]. One-Step Ultrafiltration Process for Separation and Purification of a Keratinolytic Protease Produced with Feather Meal. International Journal of Chemical Engineering No. 2018 (2018), pp.1-7.
https://search.emarefa.net/detail/BIM-1170205
American Medical Association (AMA)
Machado, Juliana R.& Severo, Emanuelle E.& de Oliveira, Janaina M. G.& Ores, Joana da C.& Brandelli, Adriano& Kalil, Susana J.. One-Step Ultrafiltration Process for Separation and Purification of a Keratinolytic Protease Produced with Feather Meal. International Journal of Chemical Engineering. 2018. Vol. 2018, no. 2018, pp.1-7.
https://search.emarefa.net/detail/BIM-1170205
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1170205
