Development of a Novel, Sensitive, Selective, and Fast Methodology to Determine Malondialdehyde in Leaves of Melon Plants by Ultra-High-Performance Liquid Chromatography-Tandem Mass Spectrometry
Joint Authors
Yonny, Melisa E.
Rodríguez Torressi, Ariel
Nazareno, Mónica A.
Cerutti, Soledad
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-01-19
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Early production of melon plant (Cucumis melo) is carried out using tunnels structures, where extreme temperatures lead to high reactive oxygen species production and, hence, oxidative stress.
Malondialdehyde (MDA) is a recognized biomarker of the advanced oxidative status in a biological system.
Thus a reliable, sensitive, simple, selective, and rapid separative strategy based on ultra-high-performance liquid chromatography coupled to positive electrospray-tandem mass spectrometry (UPLC-(+)ESI-MS/MS) was developed for the first time to measure MDA, without derivatization, in leaves of melon plants exposed to stress conditions.
The detection and quantitation limits were 0.02 μg·L−1 and 0.08 μg·L−1, respectively, which was demonstrated to be better than the methodologies currently reported in the literature.
The accuracy values were between 96% and 104%.
The precision intraday and interday values were 2.7% and 3.8%, respectively.
The optimized methodology was applied to monitoring of changes in MDA levels between control and exposed to thermal stress conditions melon leaves samples.
Important preliminary conclusions were obtained.
Besides, a comparison between MDA levels in melon leaves quantified by the proposed method and the traditional thiobarbituric acid reactive species (TBARS) approach was undertaken.
The MDA determination by TBARS could lead to unrealistic conclusions regarding the oxidative stress status in plants.
American Psychological Association (APA)
Yonny, Melisa E.& Rodríguez Torressi, Ariel& Nazareno, Mónica A.& Cerutti, Soledad. 2017. Development of a Novel, Sensitive, Selective, and Fast Methodology to Determine Malondialdehyde in Leaves of Melon Plants by Ultra-High-Performance Liquid Chromatography-Tandem Mass Spectrometry. Journal of Analytical Methods in Chemistry،Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1170206
Modern Language Association (MLA)
Yonny, Melisa E.…[et al.]. Development of a Novel, Sensitive, Selective, and Fast Methodology to Determine Malondialdehyde in Leaves of Melon Plants by Ultra-High-Performance Liquid Chromatography-Tandem Mass Spectrometry. Journal of Analytical Methods in Chemistry No. 2017 (2017), pp.1-9.
https://search.emarefa.net/detail/BIM-1170206
American Medical Association (AMA)
Yonny, Melisa E.& Rodríguez Torressi, Ariel& Nazareno, Mónica A.& Cerutti, Soledad. Development of a Novel, Sensitive, Selective, and Fast Methodology to Determine Malondialdehyde in Leaves of Melon Plants by Ultra-High-Performance Liquid Chromatography-Tandem Mass Spectrometry. Journal of Analytical Methods in Chemistry. 2017. Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1170206
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1170206