UPLC-Tandem Mass Spectrometry Method for Simultaneous Determination of Fluoxetine, Risperidone, and Its Active Metabolite 9-Hydroxyrisperidone in Plasma: Application to Pharmacokinetics Study in Rats
Joint Authors
Tammam, Marwa
Abo Talib, Nisreen F.
Ezzeldin, Essam
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-06-01
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Risperidone (RIS) is used as an antipsychotic drug alone or with other drugs, like fluoxetine (FLX).
A simple method was developed and validated for the determination of both RIS and its metabolite 9-hydroxyrisperidone (9-OH-RIS), FLX, and olanzapine (OLA) as an internal standard in rat’s plasma using UPLC-MS/MS.
FLX, RIS, 9-OH-RIS, and OLA were purified using acetonitrile as a protein precipitating agent.
Separation was performed on an ACQUITY™ “UPLC BEH™” C18 column (50 mm × 2.1 mm i.d., 1.7 μm; Waters Corp., USA).
The ranges of the calibration curves were 1.0–1000.0 ng/mL for FLX and 0.2–1000.0 ng/mL for RIS and 9-OH-RIS.
Linearity, recovery, precision, and stability were within the acceptable range.
This method is rapid, fast, and precise for the determination of RIS and FLX in plasma and is applicable in pharmacokinetic studies.
American Psychological Association (APA)
Ezzeldin, Essam& Abo Talib, Nisreen F.& Tammam, Marwa. 2017. UPLC-Tandem Mass Spectrometry Method for Simultaneous Determination of Fluoxetine, Risperidone, and Its Active Metabolite 9-Hydroxyrisperidone in Plasma: Application to Pharmacokinetics Study in Rats. Journal of Analytical Methods in Chemistry،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1170226
Modern Language Association (MLA)
Ezzeldin, Essam…[et al.]. UPLC-Tandem Mass Spectrometry Method for Simultaneous Determination of Fluoxetine, Risperidone, and Its Active Metabolite 9-Hydroxyrisperidone in Plasma: Application to Pharmacokinetics Study in Rats. Journal of Analytical Methods in Chemistry No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1170226
American Medical Association (AMA)
Ezzeldin, Essam& Abo Talib, Nisreen F.& Tammam, Marwa. UPLC-Tandem Mass Spectrometry Method for Simultaneous Determination of Fluoxetine, Risperidone, and Its Active Metabolite 9-Hydroxyrisperidone in Plasma: Application to Pharmacokinetics Study in Rats. Journal of Analytical Methods in Chemistry. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1170226
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1170226