Quantitative Analysis of the Proteome and the Succinylome in the Thyroid Tissue of High-Fat Diet-Induced Hypothyroxinemia in Rats
Joint Authors
Gao, Ling
Zhao, Jiajun
Shao, Shanshan
Yu, Chunxiao
Zhao, Meng
Zhao, Lifang
Hu, Baoxiang
Luo, Dandan
Yang, Yashuang
Zhang, Xiaohan
Source
International Journal of Endocrinology
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-07-23
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
Hypothyroidism is a common disease, and its molecular mechanism still needs further investigation.
Lysine succinylation is found to be involved in various metabolic processes associated with hypothyroidism.
We performed quantitative analysis on lysine succinylome in thyroids of rats with hypothyroxinemia, which was induced through the administration of a high-fat diet.
Overall, 129 differentially expressed proteins were quantified.
Downregulated proteins were enriched in the thyroid hormone synthesis and thyroid hormone signaling pathways and were mainly localized in the mitochondria.
In addition, 172 lysine succinylation sites on 104 proteins were obviously changed.
Decreased succinylated proteins were involved in diverse metabolic pathways and were primarily localized in mitochondria.
Finally, the mitochondrial oxygen consumption rates of human normal thyroid epithelial cells were measured to further verify the role of lysine succinylation.
The mitochondrial oxygen consumption rates were markedly blunted in the cells treated with palmitic acid (all p<0.05), and the changes were reversed when the cells were treated with palmitic acid and desuccinylase inhibitor together (all p<0.05).
Thus, we theorize that the thyroid differentially expressed proteins and changed succinylation levels played potential roles in the mitochondria-mediated energy metabolism in the high-fat diet-induced hypothyroxinemia rat model.
American Psychological Association (APA)
Hu, Baoxiang& Zhao, Meng& Luo, Dandan& Yu, Chunxiao& Shao, Shanshan& Zhao, Lifang…[et al.]. 2020. Quantitative Analysis of the Proteome and the Succinylome in the Thyroid Tissue of High-Fat Diet-Induced Hypothyroxinemia in Rats. International Journal of Endocrinology،Vol. 2020, no. 2020, pp.1-15.
https://search.emarefa.net/detail/BIM-1170227
Modern Language Association (MLA)
Hu, Baoxiang…[et al.]. Quantitative Analysis of the Proteome and the Succinylome in the Thyroid Tissue of High-Fat Diet-Induced Hypothyroxinemia in Rats. International Journal of Endocrinology No. 2020 (2020), pp.1-15.
https://search.emarefa.net/detail/BIM-1170227
American Medical Association (AMA)
Hu, Baoxiang& Zhao, Meng& Luo, Dandan& Yu, Chunxiao& Shao, Shanshan& Zhao, Lifang…[et al.]. Quantitative Analysis of the Proteome and the Succinylome in the Thyroid Tissue of High-Fat Diet-Induced Hypothyroxinemia in Rats. International Journal of Endocrinology. 2020. Vol. 2020, no. 2020, pp.1-15.
https://search.emarefa.net/detail/BIM-1170227
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1170227