Cell Disruption of Chaetoceros calcitrans by Microwave and Ultrasound in Lipid Extraction
Joint Authors
Nogueira, Daniela Almeida
da Silveira, Juliane Machado
Vidal, Évelin Mendes
Ribeiro, Natália Torres
Veiga Burkert, Carlos André
Source
International Journal of Chemical Engineering
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-09-02
Country of Publication
Egypt
No. of Pages
6
Abstract EN
Downstream processing, such as cell disruption and extraction, constitutes a key step in microalgal-based industrial bioprocesses, mainly due to high costs and environmental impact.
In this context, extraction technologies need to be improved, including the use of nonconventional cell disruption techniques suitable for scale-up, such as microwave and ultrasound.
Therefore, this study aimed at investigating the effects of different methods of cell disruption (microwave and ultrasound) on lipid extraction from biomass of the diatom Chaetoceros calcitrans cultured in mixotrophic conditions in a medium with natural sea water and residual glycerol, with different treatment times.
Both techniques applied to the biomass were efficient; that is, the results were 24.6 ± 1.3% lipids (ultrasound for 5 min) and 24.2 ± 0.9% lipids (microwave for 40 s), with no significant differences between them (p≥0.05).
Likewise, there was no significant difference regarding the chemical disruption with hydrochloric acid 2 M as control (24.2 ± 1.0%).
The ultrasound method consumed less energy than the microwave method.
Both cell disruption methods applied to the biomass resulted in changes in the fatty acid profiles, that is, percentages of saturated fatty acids increased from 7.7% (control) to 16.6% (microwave) and 15.5% (ultrasound), whereas polyunsaturated ones increased from 12.8% (control) to 22.8% (microwave) and 21.8% (ultrasound).
Concerning monounsaturated fatty acids, percentages decreased from 79.5% (control) to 60.6% (microwave) and 62.7% (ultrasound).
American Psychological Association (APA)
Nogueira, Daniela Almeida& da Silveira, Juliane Machado& Vidal, Évelin Mendes& Ribeiro, Natália Torres& Veiga Burkert, Carlos André. 2018. Cell Disruption of Chaetoceros calcitrans by Microwave and Ultrasound in Lipid Extraction. International Journal of Chemical Engineering،Vol. 2018, no. 2018, pp.1-6.
https://search.emarefa.net/detail/BIM-1170256
Modern Language Association (MLA)
Nogueira, Daniela Almeida…[et al.]. Cell Disruption of Chaetoceros calcitrans by Microwave and Ultrasound in Lipid Extraction. International Journal of Chemical Engineering No. 2018 (2018), pp.1-6.
https://search.emarefa.net/detail/BIM-1170256
American Medical Association (AMA)
Nogueira, Daniela Almeida& da Silveira, Juliane Machado& Vidal, Évelin Mendes& Ribeiro, Natália Torres& Veiga Burkert, Carlos André. Cell Disruption of Chaetoceros calcitrans by Microwave and Ultrasound in Lipid Extraction. International Journal of Chemical Engineering. 2018. Vol. 2018, no. 2018, pp.1-6.
https://search.emarefa.net/detail/BIM-1170256
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1170256