LC-MS-Based Metabolic Fingerprinting of Aqueous Humor
Joint Authors
Mariak, Zofia
Ciborowski, Michal
Pietrowska, Karolina
Dmuchowska, Diana Anna
Samczuk, Paulina
Kowalczyk, Tomasz
Krasnicki, Pawel
Wojnar, Malgorzata
Skowronska, Aleksandra
Kretowski, Adam
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-01-05
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Aqueous humor (AH) is a transparent fluid which fills the anterior and posterior chambers of the eye.
It supplies nutrients and removes metabolic waste from avascular tissues in the eye.
Proper homeostasis of AH is required to maintain adequate intraocular pressure as well as optical and refractive properties of the eye.
Application of metabolomics to study human AH may improve knowledge about the molecular mechanisms of eye diseases.
Until now, global analysis of metabolites in AH has been mainly performed using NMR.
Among the analytical platforms used in metabolomics, LC-MS allows for the highest metabolome coverage.
The aim of this study was to develop a method for extraction and analysis of AH metabolites by LC-QTOF-MS.
Different protocols for AH preparation were tested.
The best results were obtained when one volume of AH was mixed with one volume of methanol : ethanol (1 : 1).
In the final method, 2 µL of extracted sample was analyzed by LC-QTOF-MS.
The method allowed for reproducible measurement of over 1000 metabolic features.
Almost 250 metabolites were identified in AH and assigned to 47 metabolic pathways.
This method is suitable to study the potential role of amino acids, lipids, oxidative stress, or microbial metabolites in development of ocular diseases.
American Psychological Association (APA)
Pietrowska, Karolina& Dmuchowska, Diana Anna& Samczuk, Paulina& Kowalczyk, Tomasz& Krasnicki, Pawel& Wojnar, Malgorzata…[et al.]. 2017. LC-MS-Based Metabolic Fingerprinting of Aqueous Humor. Journal of Analytical Methods in Chemistry،Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1170268
Modern Language Association (MLA)
Pietrowska, Karolina…[et al.]. LC-MS-Based Metabolic Fingerprinting of Aqueous Humor. Journal of Analytical Methods in Chemistry No. 2017 (2017), pp.1-13.
https://search.emarefa.net/detail/BIM-1170268
American Medical Association (AMA)
Pietrowska, Karolina& Dmuchowska, Diana Anna& Samczuk, Paulina& Kowalczyk, Tomasz& Krasnicki, Pawel& Wojnar, Malgorzata…[et al.]. LC-MS-Based Metabolic Fingerprinting of Aqueous Humor. Journal of Analytical Methods in Chemistry. 2017. Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1170268
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1170268