Simultaneous Determination of Dexamethasone, Ondansetron, Granisetron, Tropisetron, and Azasetron in Infusion Samples by HPLC with DAD Detection
Joint Authors
Chen, Fu-chao
Wang, Lin-hai
Guo, Jun
Shi, Xiao-ya
Fang, Bao-xia
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-01-11
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
A simple and rapid high-performance liquid chromatography with diode array detector (HPLC-DAD) method has been developed and validated for simultaneous quantification of five antiemetic agents in infusion samples: dexamethasone, ondansetron, granisetron, tropisetron, and azasetron.
The chromatographic separation was achieved on a Phenomenex C18 column (4.6 mm × 150 mm, 5 μm) using acetonitrile-50 mM KH2PO4 buffer-triethylamine (25 : 74 : 1; v/v; pH 4.0).
Flow rate was 1.0 mL/min with a column temperature of 30°C.
Validation of the method was made in terms of specificity, linearity, accuracy, and intra- and interday precision, as well as quantification and detection limits.
The developed method can be used in the laboratory to routinely quantify dexamethasone, ondansetron, granisetron, tropisetron, and azasetron simultaneously and to evaluate the physicochemical stability of referred drugs in mixtures for endovenous use.
American Psychological Association (APA)
Chen, Fu-chao& Wang, Lin-hai& Guo, Jun& Shi, Xiao-ya& Fang, Bao-xia. 2017. Simultaneous Determination of Dexamethasone, Ondansetron, Granisetron, Tropisetron, and Azasetron in Infusion Samples by HPLC with DAD Detection. Journal of Analytical Methods in Chemistry،Vol. 2017, no. 2017, pp.1-7.
https://search.emarefa.net/detail/BIM-1170269
Modern Language Association (MLA)
Chen, Fu-chao…[et al.]. Simultaneous Determination of Dexamethasone, Ondansetron, Granisetron, Tropisetron, and Azasetron in Infusion Samples by HPLC with DAD Detection. Journal of Analytical Methods in Chemistry No. 2017 (2017), pp.1-7.
https://search.emarefa.net/detail/BIM-1170269
American Medical Association (AMA)
Chen, Fu-chao& Wang, Lin-hai& Guo, Jun& Shi, Xiao-ya& Fang, Bao-xia. Simultaneous Determination of Dexamethasone, Ondansetron, Granisetron, Tropisetron, and Azasetron in Infusion Samples by HPLC with DAD Detection. Journal of Analytical Methods in Chemistry. 2017. Vol. 2017, no. 2017, pp.1-7.
https://search.emarefa.net/detail/BIM-1170269
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1170269