The Evidence of Cooperative Binding of a Ligand to G4 DNA
Author
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-10-18
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Intrinsic constants of the ligand binding with G4 DNA (guanine-rich DNA sequence) using quantitative standards can be convenient providing the assessment for elucidating the possibility of such structures participation in biochemical processes.
In the present communication, the hard + soft modelling approach to calculate intrinsic constants of a ligand binding with short DNA molecule, particularly such as G4 DNA, has been proposed.
The suggested approach has focused upon the quantitative evaluating of a mutual influence between sites and between bound ligands.
The cross-validation between a new hard + soft modelling and conventional stepwise complex formation algorithm has been conducted.
A number of simulated examples will illustrate the methodology.
The experimental mole-ratio titration of TMPyP4 by G4 DNA [(CG3)2CGC(AG3)2G] has been reexamined.
The [(CG3)2CGC(AG3)2G] that folds from a G-rich sequence found in the promoter region of c-kit oncogene can be considered as a molecule with two equivalent mutually influence binding sites.
American Psychological Association (APA)
Kudrev, A. G.. 2017. The Evidence of Cooperative Binding of a Ligand to G4 DNA. Journal of Analytical Methods in Chemistry،Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1170274
Modern Language Association (MLA)
Kudrev, A. G.. The Evidence of Cooperative Binding of a Ligand to G4 DNA. Journal of Analytical Methods in Chemistry No. 2017 (2017), pp.1-9.
https://search.emarefa.net/detail/BIM-1170274
American Medical Association (AMA)
Kudrev, A. G.. The Evidence of Cooperative Binding of a Ligand to G4 DNA. Journal of Analytical Methods in Chemistry. 2017. Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1170274
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1170274