The Effect and Mechanism of Emodin on the NO Secretion of Human Umbilical Vein Endothelial Cells (HUVECs) Induced by High Glucose
Joint Authors
Li, Yajia
Li, Qiangxiang
Xie, Chunyan
Huang, Yanfei
Jia, Limin
Source
International Journal of Endocrinology
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-06-12
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
Objective.
To investigate the effects of emodin on nitric oxide (NO) secretion induced by high glucose in human umbilical vein endothelial cells (HUVECs) through the p-Akt signaling pathway.
Methods.
Sensitivity of cells to emodin was determined by MTT assay to establish the experimental concentrations; then, HUVECs were treated with high-dose (33.3 mmol/L) glucose (HG), HG + emodin (HG + E), HG + the Akt phosphorylation inhibitor LY294002 (HG + LY), or HG + E + LY.
The p-Akt (Ser 473) expression in 48 h was analyzed using Western blot.
NO effect on the secretion of HUVECs was analyzed using nitrate reductase assay.
Results.
The sensitive emodin concentration for HUVECs growth was 10 mol/L (P<0.05).
Compared with the HG group, NO secretion was significantly higher in the HG + E group (P<0.05), whereas it was lowest in the HG + LY group (P<0.05).
Compared with the HG + LY group, NO secretion was increased in the HG + E + LY group (P<0.05).
The p-Akt protein expression was decreased in the HG + LY group when compared to the HG group (P<0.05), while it significantly increased in the HG + E group (P<0.05).
Compared with HG + LY group, p-Akt protein expression was significantly higher in the HG + E + LY group (P<0.05).
Conclusion.
Emodin could improve the NO secretion of HUVECs by high glucose through the p-Akt signaling pathway.
American Psychological Association (APA)
Li, Yajia& Li, Qiangxiang& Xie, Chunyan& Huang, Yanfei& Jia, Limin. 2020. The Effect and Mechanism of Emodin on the NO Secretion of Human Umbilical Vein Endothelial Cells (HUVECs) Induced by High Glucose. International Journal of Endocrinology،Vol. 2020, no. 2020, pp.1-5.
https://search.emarefa.net/detail/BIM-1170323
Modern Language Association (MLA)
Li, Yajia…[et al.]. The Effect and Mechanism of Emodin on the NO Secretion of Human Umbilical Vein Endothelial Cells (HUVECs) Induced by High Glucose. International Journal of Endocrinology No. 2020 (2020), pp.1-5.
https://search.emarefa.net/detail/BIM-1170323
American Medical Association (AMA)
Li, Yajia& Li, Qiangxiang& Xie, Chunyan& Huang, Yanfei& Jia, Limin. The Effect and Mechanism of Emodin on the NO Secretion of Human Umbilical Vein Endothelial Cells (HUVECs) Induced by High Glucose. International Journal of Endocrinology. 2020. Vol. 2020, no. 2020, pp.1-5.
https://search.emarefa.net/detail/BIM-1170323
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1170323