Simultaneous Determination of 11 Compounds in Gualou Guizhi Granule and Pharmacokinetics Study by UPLC-MSMS
Joint Authors
Sun, Chengtao
Xu, Wei
Xu, Wen
Zhang, Yuqin
Yu, Lishuang
Ye, Miao
Chu, Kedan
Lin, Yu
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-07-20
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
A rapid and sensitive ultrafast performance liquid chromatography-tandem mass spectrometry method (UPLC-MS/MS) was developed for the simultaneous determination of 11 compounds in Gualou Guizhi Granule (GLGZG), including liquiritin, isoliquiritin, liquirtin apioside, isoliquiritin apioside, liquiritigenin, isoliquiritigenin, glycyrrhizic acid, glycyrrhetinic acid, paeoniflorin, albiflorin, and paeoniflorin sulfonate in rat plasma.
UPLC-MS/MS assay with negative ion mode was performed on a Waters CORTECS C18 (2.1 × 100 mm, 1.6 μm) with the mobile phase consisting of 0.1% aqueous formic acid (A) and acetonitrile (B) in gradient elution at a flow rate of 0.25 mL·min−1.
The method was linear for all analytes within the detection range (r≥0.9597).
The inter- and intraday precision (RSD) were 2.21–6.41% and 1.67–6.18%; the inter- and intraday accuracy (recover) were 92.48–114.03% and 90.23–112.04%.
And the recovery rate ranged from 81.30% to 108.22%.
The matrix effect values obtained for analytes ranged from 88.91% to 113.32%.
This validated method was successfully applied to a pharmacokinetics study in rats after oral administration of GLGZG.
American Psychological Association (APA)
Sun, Chengtao& Xu, Wen& Zhang, Yuqin& Yu, Lishuang& Ye, Miao& Chu, Kedan…[et al.]. 2017. Simultaneous Determination of 11 Compounds in Gualou Guizhi Granule and Pharmacokinetics Study by UPLC-MSMS. Journal of Analytical Methods in Chemistry،Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1170331
Modern Language Association (MLA)
Sun, Chengtao…[et al.]. Simultaneous Determination of 11 Compounds in Gualou Guizhi Granule and Pharmacokinetics Study by UPLC-MSMS. Journal of Analytical Methods in Chemistry No. 2017 (2017), pp.1-13.
https://search.emarefa.net/detail/BIM-1170331
American Medical Association (AMA)
Sun, Chengtao& Xu, Wen& Zhang, Yuqin& Yu, Lishuang& Ye, Miao& Chu, Kedan…[et al.]. Simultaneous Determination of 11 Compounds in Gualou Guizhi Granule and Pharmacokinetics Study by UPLC-MSMS. Journal of Analytical Methods in Chemistry. 2017. Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1170331
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1170331