Comparative Liquid Chromatographic Study for Concurrent Determination of Canagliflozin and Metformin in Combined Tablets
Joint Authors
Zaghary, Wafaa A.
Mowaka, Shereen
Hendy, Moataz S.
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-12-03
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
New HPLC-UV method (method A), for simultaneous determination of metformin (MET) and canagliflozin (CANA), was developed and compared to another novel UPLC-UV method (method B) in their tablet combination.
Concerning method A, isocratic separation was done by C18 column (100 mm × 2.1 mm, 3 μm) using methanol and 0.03 M phosphate buffer (75:25, v/v) at pH 3.2 as a mobile phase.
Meanwhile, chromatographic separation in method B was achieved via Hypersil® gold (50 mm × 2.1 mm, 1.9 μm).
Mobile phase was methanol and 0.03 M phosphate buffer at ratio of 80:20 v/v.
In both, detection was done at wavelength of 240 nm.
Method A showed satisfactory linearity results over 1–50 μg·mL−1 and 0.5–100 μg·mL−1, while method B linearity was at 0.1–50 μg·mL−1 and 0.25–100 μg·mL−1 for CANA and MET, respectively.
In terms of accuracy and precision, method A accuracy was 99.81±0.73 and 99.37±0.54, while method B gave accuracy of 99.47±1.03 and 99.73±0.89 for CANA and MET, respectively.
For precision, the % RSD was found to be less than 2% for three concentrations analyzed three times.
The two methods are convenient for quality laboratories, yet the UPLC method offered the advantage of shorter run times and higher sensitivity.
American Psychological Association (APA)
Zaghary, Wafaa A.& Mowaka, Shereen& Hendy, Moataz S.. 2017. Comparative Liquid Chromatographic Study for Concurrent Determination of Canagliflozin and Metformin in Combined Tablets. Journal of Analytical Methods in Chemistry،Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1170347
Modern Language Association (MLA)
Zaghary, Wafaa A.…[et al.]. Comparative Liquid Chromatographic Study for Concurrent Determination of Canagliflozin and Metformin in Combined Tablets. Journal of Analytical Methods in Chemistry No. 2017 (2017), pp.1-9.
https://search.emarefa.net/detail/BIM-1170347
American Medical Association (AMA)
Zaghary, Wafaa A.& Mowaka, Shereen& Hendy, Moataz S.. Comparative Liquid Chromatographic Study for Concurrent Determination of Canagliflozin and Metformin in Combined Tablets. Journal of Analytical Methods in Chemistry. 2017. Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1170347
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1170347