Microextraction by Packed Molecularly Imprinted Polymer Combined Ultra-High-Performance Liquid Chromatography for the Determination of Levofloxacin in Human Plasma
Joint Authors
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-01-21
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Fluoroquinolones are considered as gold standard for the prevention of bacterial infections.
To improve assessment of antibacterial efficacy, a novel method for determination of levofloxacin was developed and validated.
Deep eutectic solvents (DESs) as only green solvent were used as a porogen for preparation of water-compatible molecularly imprinted polymers (MIPs) with a pseudotemplate.
The DESs-MIPs were characterized in detail, including scanning electron microscope, nitrogen sorption porosimetry, and Fourier transform-infrared spectra.
Clearly, the maximum binding capacity of levofloxacin on DESs-MIPs in water and methanol was 0.216 and 0.077 μmol g−1, respectively.
The DESs-MIPs as adsorbing materials were applied in microextraction by packed sorbent (MEPS), and the DESs-MIPs-MEPS conditions were optimized.
The DESs-MIPs-MEPS coupled with ultra-high-performance liquid chromatography (UHPLC) was used to determine levofloxacin in human plasma.
The method was found linear over 0.05–10 μg mL−1 with coefficient of correlation equal to 0.9988.
The limit of detection and limit of quantification were 0.012 and 0.04 μg mL−1, respectively.
At three spiked levels, the precision of proposed method was between 95.3% and 99.7% with intraday and interday relative standard deviations ≤8.9%.
Finally, the developed method was used to examine levofloxacin from human plasma of 20 hospitalized patients after transrectal ultrasound-guided prostate biopsy, and the average concentration (±SD) of levofloxacin was 2.35 ± 0.99 μg mL−1 in plasma.
American Psychological Association (APA)
Meng, Jia& Wang, Xu. 2019. Microextraction by Packed Molecularly Imprinted Polymer Combined Ultra-High-Performance Liquid Chromatography for the Determination of Levofloxacin in Human Plasma. Journal of Chemistry،Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1171104
Modern Language Association (MLA)
Meng, Jia& Wang, Xu. Microextraction by Packed Molecularly Imprinted Polymer Combined Ultra-High-Performance Liquid Chromatography for the Determination of Levofloxacin in Human Plasma. Journal of Chemistry No. 2019 (2019), pp.1-9.
https://search.emarefa.net/detail/BIM-1171104
American Medical Association (AMA)
Meng, Jia& Wang, Xu. Microextraction by Packed Molecularly Imprinted Polymer Combined Ultra-High-Performance Liquid Chromatography for the Determination of Levofloxacin in Human Plasma. Journal of Chemistry. 2019. Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1171104
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1171104