Detection of L-Proline-Catalyzed Michael Addition Reaction in Model Biomembrane
Joint Authors
Suga, Keishi
Hirose, Masanori
Sugisaki, Shigenori
Umakoshi, Hiroshi
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-02-26
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
A method to detect the L-proline- (L-Pro-) catalyzed Michael addition reaction in model biomembranes has been established, using N-[p(2-benzimidazolyl)phenyl]maleimide and acetone as reactants.
The effect of liposome membranes on this reaction was kinetically analyzed using fluorescence spectroscopy.
The kinetics of the reaction were different from those of the constituent lipids of the liposomes.
Zwitterionic 1,2-dipalmitoyl-sn-glycero-3-phosphocholine liposome, which is in the solid-ordered phase, had a better value of reaction rate, suggesting that the reaction rate constants of this reaction in liposome membrane systems could be regulated by the characteristics of the liposome membrane (i.e., the phase state and surface charge).
Based on the results obtained, a plausible model of the L-Pro-catalyzed Michael addition reaction was discussed.
The obtained results provide us with an easily detectable method to assess the reactivity of L-Pro in biological systems.
American Psychological Association (APA)
Hirose, Masanori& Sugisaki, Shigenori& Suga, Keishi& Umakoshi, Hiroshi. 2019. Detection of L-Proline-Catalyzed Michael Addition Reaction in Model Biomembrane. Journal of Chemistry،Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1171137
Modern Language Association (MLA)
Hirose, Masanori…[et al.]. Detection of L-Proline-Catalyzed Michael Addition Reaction in Model Biomembrane. Journal of Chemistry No. 2019 (2019), pp.1-8.
https://search.emarefa.net/detail/BIM-1171137
American Medical Association (AMA)
Hirose, Masanori& Sugisaki, Shigenori& Suga, Keishi& Umakoshi, Hiroshi. Detection of L-Proline-Catalyzed Michael Addition Reaction in Model Biomembrane. Journal of Chemistry. 2019. Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1171137
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1171137