Whole Genome 5′-Methylcytosine Level Quantification in Cirrhotic HCV-Infected Egyptian Patients with and without Hepatocellular Carcinoma
Joint Authors
Awad, Ahmed M.
Ragab, Wafaa S.
Degheidy, Nourhan
Ooda, Said Ahmed
Source
International Journal of Genomics
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-10-05
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
DNA methylation is an epigenetic mechanism used by cells to control gene expression.
DNA methylation is a commonly used epigenetic signaling tool that can hold genes in the “off” position.
Chronic infection with hepatitis C virus (HCV) is considered a major risk for chronic liver impairment.
It is the most common leading cause of HCC.
The present work is aimed at studying whole genome 5′-methylcytosine levels in cirrhotic HCV-infected Egyptian patients.
In the present study, 120 Egyptian adults were included.
They were divided into two groups: group І (40 apparently healthy control subjects) and group ІІ (80 HCV-infected patients).
Furthermore, group II was subdivided into 2 subgroups according to the presence of HCC in HCV-infected subjects.
To all studied subjects, the level of 5-mC% was measured in peripheral blood.
In the present study, the median of 5′-methylcytosine% in the control group (group I) was 2.5, in the HCV group (group IIa) was 2.45, and in the HCC group (group II b) was 2.25.
A stepwise decrease in 5′-methylcytosine% from the control (group I) toward HCC (group IIb) was observed, taking into consideration that the stepwise global hypomethylation was not statistically significant (p=0.811).
There was a negative correlation between ALT and 5′-methylcytosine% (p=−0.029).
From this study, we can conclude that global DNA 5′-methylcytosine% does not differ in HCV-infected cirrhotic patients and HCC patients when compared to normal controls.
Consecutively, we had concluded that there is no impact of 5′-methylcytosine% on the development of liver cirrhosis or HCC.
Moreover, the negative correlation between 5′-methylcytosine% and serum ALT level denotes a trend of decrease in 5′-methylcytosine% with more liver damage.
American Psychological Association (APA)
Awad, Ahmed M.& Ragab, Wafaa S.& Degheidy, Nourhan& Ooda, Said Ahmed. 2020. Whole Genome 5′-Methylcytosine Level Quantification in Cirrhotic HCV-Infected Egyptian Patients with and without Hepatocellular Carcinoma. International Journal of Genomics،Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1171185
Modern Language Association (MLA)
Awad, Ahmed M.…[et al.]. Whole Genome 5′-Methylcytosine Level Quantification in Cirrhotic HCV-Infected Egyptian Patients with and without Hepatocellular Carcinoma. International Journal of Genomics No. 2020 (2020), pp.1-8.
https://search.emarefa.net/detail/BIM-1171185
American Medical Association (AMA)
Awad, Ahmed M.& Ragab, Wafaa S.& Degheidy, Nourhan& Ooda, Said Ahmed. Whole Genome 5′-Methylcytosine Level Quantification in Cirrhotic HCV-Infected Egyptian Patients with and without Hepatocellular Carcinoma. International Journal of Genomics. 2020. Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1171185
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1171185