Diagnostic Value of miR-103 in Patients with Sepsis and Noninfectious SIRS and Its Regulatory Role in LPS-Induced Inflammatory Response by Targeting TLR4
Joint Authors
Source
International Journal of Genomics
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-05-13
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Background.
Sepsis is a life-threatening condition and a systemic inflammatory response syndrome (SIRS) driven by infection.
This study aimed at investigating the expression of microRNA-103 (miR-103) in sepsis patients, evaluating its diagnostic value, and exploring the regulatory effect of miR-103 on LPS-induced inflammation in monocytes.
Methods.
Expression of miR-103 was measured using quantitative real-time PCR.
A receiver operating characteristics curve was plotted to evaluate the diagnostic vale of miR-103.
Serum and cell supernatant levels of proinflammatory cytokines were analyzed using ELISA.
The interaction between miR-103 and Toll-like receptors 4 (TLR4) was analyzed using luciferase reporter assay.
The effect of miR-103 on inflammation was examined in LPS-treated monocytes.
Results.
Serum expression of miR-103 was decreased in noninfectious SIRS and sepsis patients compared with healthy controls, and the lowest expression value was observed in sepsis patients (all P<0.05).
Serum levels of miR-103 have considerable diagnostic accuracy in distinguishing sepsis patients from SIRS patients and healthy controls.
A negative correlation was found between miR-103 and inflammatory responses in sepsis patients.
TLR4 was demonstrated to be a direct target of miR-103 and was negatively regulated by miR-103 in monocytes.
The promoted inflammatory responses by LPS in monocytes were reversed by the overexpression of miR-103.
Conclusion.
All the data revealed that serum decreased miR-103 in sepsis patients serves as a promising noninvasive diagnostic biomarker and may be involved in the pathogenesis of sepsis by regulating inflammatory responses via targeting TLR4.
American Psychological Association (APA)
Yang, Min& Zhao, Li& Sun, Mingyan. 2020. Diagnostic Value of miR-103 in Patients with Sepsis and Noninfectious SIRS and Its Regulatory Role in LPS-Induced Inflammatory Response by Targeting TLR4. International Journal of Genomics،Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1171193
Modern Language Association (MLA)
Yang, Min…[et al.]. Diagnostic Value of miR-103 in Patients with Sepsis and Noninfectious SIRS and Its Regulatory Role in LPS-Induced Inflammatory Response by Targeting TLR4. International Journal of Genomics No. 2020 (2020), pp.1-8.
https://search.emarefa.net/detail/BIM-1171193
American Medical Association (AMA)
Yang, Min& Zhao, Li& Sun, Mingyan. Diagnostic Value of miR-103 in Patients with Sepsis and Noninfectious SIRS and Its Regulatory Role in LPS-Induced Inflammatory Response by Targeting TLR4. International Journal of Genomics. 2020. Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1171193
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1171193