Characterization of an IncR Plasmid with Two Copies of ISCR-Linked qnrB6 from ST968 Klebsiella pneumoniae
Joint Authors
Lin, Xi
Lu, Junwan
Qian, Changrui
Chen, Qianqian
Shen, Kai
Li, Kewei
Zhou, Wangxiao
Zhu, Xinyi
Zhou, Danying
Sun, Zhewei
Liu, Hongmao
Xu, Teng
Lu, Shunfei
Bao, Qiyu
Lu, Wei
Source
International Journal of Genomics
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-11-24
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
To characterize the molecular structure of IncR plasmid-related sequences, comparative genomic analysis was conducted using 261 IncR plasmid backbone-related sequences.
Among the sequences, 257 were IncR plasmids including the multidrug-resistance IncR plasmid pR50-74 from Klebsiella pneumoniae strain R50 of this work, and the other four were from bacterial chromosomes.
The IncR plasmids were derived from different bacterial genera or species, mainly Klebsiella pneumoniae (70.82%, 182/257), Escherichia coli (11.28%, 29/257), Enterobacter cloacae (7.00%, 18/257), and Citrobacter freundii (3.50%, 9/257).
The bacterial chromosomes carrying IncR plasmid backbone sequences were derived from Proteus mirabilis AOUC-001 and Klebsiella pneumoniae KPN1344, among others.
The IncR backbone sequence of P.
mirabilis AOUC-001 chromosome shows the highest identity with that of pR50-74.
Complex class 1 integrons carrying various copies of ISCR1-sdr-qnrB6-△qacE/sul1 (ISCR1-linked qnrB6 unit) were identified in IncR plasmids.
In addition to two consecutive copies of qnrB6-qacE-sul1, the other resistance genes encoded on pR50-74 are all related to mobile genetic elements, such as IS1006, IS26, and the class 1 integron.
This study provides a clear understanding of the mobility and plasticity of the IncR plasmid backbone sequence and emphasizes the important role of ISCR in the recruitment of multicopy resistance genes.
American Psychological Association (APA)
Qian, Changrui& Zhu, Xinyi& Lu, Junwan& Shen, Kai& Chen, Qianqian& Zhou, Wangxiao…[et al.]. 2020. Characterization of an IncR Plasmid with Two Copies of ISCR-Linked qnrB6 from ST968 Klebsiella pneumoniae. International Journal of Genomics،Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1171222
Modern Language Association (MLA)
Qian, Changrui…[et al.]. Characterization of an IncR Plasmid with Two Copies of ISCR-Linked qnrB6 from ST968 Klebsiella pneumoniae. International Journal of Genomics No. 2020 (2020), pp.1-8.
https://search.emarefa.net/detail/BIM-1171222
American Medical Association (AMA)
Qian, Changrui& Zhu, Xinyi& Lu, Junwan& Shen, Kai& Chen, Qianqian& Zhou, Wangxiao…[et al.]. Characterization of an IncR Plasmid with Two Copies of ISCR-Linked qnrB6 from ST968 Klebsiella pneumoniae. International Journal of Genomics. 2020. Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1171222
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1171222