Rapid Separation of All Four Tocopherol Homologues in Selected Fruit Seeds via Supercritical Fluid Chromatography Using a Solid-Core C18 Column
Joint Authors
Urvaka, Elise
Mišina, Inga
Soliven, Arianne
Górnaś, Paweł
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-01-16
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Tocopherol separations employing the same Kinetex™ C18 column via supercritical fluid chromatography (SFC) and reversed-phase liquid chromatography (RP-LC) were compared.
The application of the SFC system with UV diode array detection (DAD) resulted in rapid separation of all four tocopherol homologues with a total analysis time below 2 min.
The RP-LC approach could not separate the isomers β and γ.
The developed SFC-DAD method was precise, accurate, and most importantly more environmentally friendlier compared to the RP-LC method due to the 125-fold decrease in methanol consumption.
The present study illustrated the selectivity differences between LC and SFC and how the C18 column can be used for tocopherol characterization.
The optimized SFC method was successfully applied for the tocopherol determination in the seeds of nine different fruit species.
American Psychological Association (APA)
Urvaka, Elise& Mišina, Inga& Soliven, Arianne& Górnaś, Paweł. 2019. Rapid Separation of All Four Tocopherol Homologues in Selected Fruit Seeds via Supercritical Fluid Chromatography Using a Solid-Core C18 Column. Journal of Chemistry،Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1171225
Modern Language Association (MLA)
Urvaka, Elise…[et al.]. Rapid Separation of All Four Tocopherol Homologues in Selected Fruit Seeds via Supercritical Fluid Chromatography Using a Solid-Core C18 Column. Journal of Chemistry No. 2019 (2019), pp.1-10.
https://search.emarefa.net/detail/BIM-1171225
American Medical Association (AMA)
Urvaka, Elise& Mišina, Inga& Soliven, Arianne& Górnaś, Paweł. Rapid Separation of All Four Tocopherol Homologues in Selected Fruit Seeds via Supercritical Fluid Chromatography Using a Solid-Core C18 Column. Journal of Chemistry. 2019. Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1171225
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1171225
