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Effects of Inbreeding on Genetic Characteristic, Growth, Survival Rates, and Immune Responses of a New Inbred Line of Exopalaemon carinicauda
Joint Authors
Wang, Jiajia
Li, Jitao
Ge, Qianqian
Chen, Zhao
Li, Jian
Source
International Journal of Genomics
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-01-04
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
The Exopalaemon carinicauda could be a useful crustacean laboratory animal in many research fields.
We newly established an inbred line of Exopalaemon carinicauda named EC4 inbred line by brother×sister mating and keeping to F11 generation.
Trends in heterozygosity in the process of producing EC4 inbred line were examined through the characterization of polymorphisms based on gene frequencies of SNP and EST-SSR loci.
The results demonstrated that the number of alleles (N), observed heterozygosity (Ho), expected heterozygosity (He), and polymorphism information content (PIC) gradually decreased with the increase of inbreeding generations.
The genetic detection results indicated that 9 (29.03%, 9/31) of the SNP loci and 15 (32.61%, 15/46) of the EST-SSR loci were homozygous in F11 generation of EC4 inbred line.
The variation of the growth-related traits, the immune responses, and antioxidant status were described in experimental full-sibling inbred populations of E.
carinicauda at five levels of inbreeding coefficient (F=0.785, F=0.816, F=0.859, F=0.886, F=0.908) under controlled laboratory conditions.
The body weight, body length, and survival rate in EC4 inbred line of all generations were less than the control population.
Inbreeding affected the antibacterial activity, phenoloxidase (PO) activity, and superoxide dismutase (SOD) which decreased at the eleventh generation of EC4 inbred line.
This study demonstrated that inbreeding had a negative effect on the economic traits and immune response, but our inbred line was established successfully until F11 and confirmed by genetic detection using SNP and EST-SSR loci.
American Psychological Association (APA)
Wang, Jiajia& Li, Jitao& Ge, Qianqian& Chen, Zhao& Li, Jian. 2020. Effects of Inbreeding on Genetic Characteristic, Growth, Survival Rates, and Immune Responses of a New Inbred Line of Exopalaemon carinicauda. International Journal of Genomics،Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1171265
Modern Language Association (MLA)
Wang, Jiajia…[et al.]. Effects of Inbreeding on Genetic Characteristic, Growth, Survival Rates, and Immune Responses of a New Inbred Line of Exopalaemon carinicauda. International Journal of Genomics No. 2020 (2020), pp.1-11.
https://search.emarefa.net/detail/BIM-1171265
American Medical Association (AMA)
Wang, Jiajia& Li, Jitao& Ge, Qianqian& Chen, Zhao& Li, Jian. Effects of Inbreeding on Genetic Characteristic, Growth, Survival Rates, and Immune Responses of a New Inbred Line of Exopalaemon carinicauda. International Journal of Genomics. 2020. Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1171265
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1171265