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Synthesis and Application of Nanomagnetic Immobilized Phospholipase C
Joint Authors
Jiang, Lianzhou
Jiang, Yang
Du, Jing
Tang, Honglin
Zhang, Xin
Li, Wenbin
Wang, Liqi
Yu, Dianyu
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-03-03
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The nanomagnetic carrier (Fe3O4@SiO2@p(GMA)) was prepared by atom transfer radical polymerization, and then, the free phospholipase C (PLC) was immobilized on it proved by the results of FT-IR analysis.
The enzyme loading was 135.64 mg/g, the enzyme activity was 8560.7 U/g, the average particle size was 99.86 ± 0.80 nm, and the specific saturation magnetization was 16.00 ± 0.20 emu/g.
PLC-Fe3O4@SiO2@p(GMA) showed the highest activities at the pH of 7.5, and tolerance temperature raised to 65°C in soybean lecithin emulsion.
Enzymatic degumming with PLC-Fe3O4@SiO2@p(GMA) under the conditions of the enzyme dosage of 110 mg/kg, reaction temperature of 65°C, pH of 7.5, and reaction time of 2.5 h resulted in residual phosphorus of 64.7 mg/kg, 1,2-diacylglycerol (1,2-DAG) contents of 1.07%, and oil yield of 98.1%.
Moreover, PLC-Fe3O4@SiO2@p(GMA) still possessed more than 80% of its initial activity after 5 cycles.
American Psychological Association (APA)
Jiang, Yang& Du, Jing& Tang, Honglin& Zhang, Xin& Li, Wenbin& Wang, Liqi…[et al.]. 2019. Synthesis and Application of Nanomagnetic Immobilized Phospholipase C. Journal of Chemistry،Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1171322
Modern Language Association (MLA)
Jiang, Yang…[et al.]. Synthesis and Application of Nanomagnetic Immobilized Phospholipase C. Journal of Chemistry No. 2019 (2019), pp.1-9.
https://search.emarefa.net/detail/BIM-1171322
American Medical Association (AMA)
Jiang, Yang& Du, Jing& Tang, Honglin& Zhang, Xin& Li, Wenbin& Wang, Liqi…[et al.]. Synthesis and Application of Nanomagnetic Immobilized Phospholipase C. Journal of Chemistry. 2019. Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1171322
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1171322