Palmitic Acid Downregulates Thyroglobulin (Tg), Sodium Iodide Symporter (NIS), and Thyroperoxidase (TPO) in Human Primary Thyrocytes: A Potential Mechanism by Which Lipotoxicity Affects Thyroid?
Joint Authors
Gao, Ling
Shao, Shanshan
Yu, Chunxiao
Zhao, Meng
Xu, Chao
Song, Yongfeng
Zhang, Xiaohan
Zhao, Jiajun
Source
International Journal of Endocrinology
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-10-17
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Our previous studies suggested that the thyroid might be a target organ affected by lipotoxicity, which might be partially related to the increasing prevalence of subclinical hypothyroidism.
However, the underlying molecular mechanism is not yet clearly established.
This study aimed to assess the effect of palmitic acid stimulation on thyrocyte function.
Upon palmitic acid stimulation, intracellular contents of lipids, as well as the expression and activity of three key molecules in thyroid hormone synthesis (i.e., thyroglobulin, sodium iodide symporter, and thyroperoxidase), were determined in human primary thyrocytes.
The contents of BODIPY® FL C16 (the fluorescently labeled palmitic acid analogue) entering into the thyrocytes were gradually increased with time extending.
Accordingly, the intracellular accumulation of both triglyceride and free fatty acids increased in dose- and time-dependent manners.
The effect of palmitic acid stimulation on thyroid hormone synthesis was then determined.
Both the mRNA and protein levels of thyroglobulin, sodium iodide symporter, and thyroperoxidase were decreased following palmitic acid stimulation.
Consistently, upon palmitic acid stimulation, the secreted thyroglobulin levels in supernatants, 131I uptake, and extracellular thyroperoxidase activity were all decreased in a dose-dependent manner.
Our results demonstrated that upon palmitic acid stimulation, the expressions of the key molecules (thyroglobulin, sodium iodide symporter, and thyroperoxidase) were reduced and their activities were suppressed, which might lead to impaired thyroid hormone synthesis.
American Psychological Association (APA)
Zhao, Meng& Zhang, Xiaohan& Gao, Ling& Song, Yongfeng& Xu, Chao& Yu, Chunxiao…[et al.]. 2018. Palmitic Acid Downregulates Thyroglobulin (Tg), Sodium Iodide Symporter (NIS), and Thyroperoxidase (TPO) in Human Primary Thyrocytes: A Potential Mechanism by Which Lipotoxicity Affects Thyroid?. International Journal of Endocrinology،Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1171342
Modern Language Association (MLA)
Zhao, Meng…[et al.]. Palmitic Acid Downregulates Thyroglobulin (Tg), Sodium Iodide Symporter (NIS), and Thyroperoxidase (TPO) in Human Primary Thyrocytes: A Potential Mechanism by Which Lipotoxicity Affects Thyroid?. International Journal of Endocrinology No. 2018 (2018), pp.1-8.
https://search.emarefa.net/detail/BIM-1171342
American Medical Association (AMA)
Zhao, Meng& Zhang, Xiaohan& Gao, Ling& Song, Yongfeng& Xu, Chao& Yu, Chunxiao…[et al.]. Palmitic Acid Downregulates Thyroglobulin (Tg), Sodium Iodide Symporter (NIS), and Thyroperoxidase (TPO) in Human Primary Thyrocytes: A Potential Mechanism by Which Lipotoxicity Affects Thyroid?. International Journal of Endocrinology. 2018. Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1171342
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1171342