Vesicle-Associated Membrane Protein-Associated Protein A Is Involved in Androgen Receptor Trafficking in Mouse Sertoli Cells
Joint Authors
Deng, Qiong
Zhang, Jianwen
Wang, Zhu
Zhang, Shengping
Zhi, Fan
Liang, Hui
Source
International Journal of Endocrinology
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-03-04
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Androgen and its receptor (AR) play an important role in maintaining spermatogenesis and male fertility.
The nonclassical androgen signaling pathway is proposed to be mediated by an AR in plasma membrane in Sertoli cells.
Our previous studies showed that testosterone induces cytoplasmic AR translocation to plasma membrane by binding with caveolin-1.
This study was conducted to the underlying molecular mechanism mediating AR trafficking.
Data from mass spectrometry using membrane coimmunoprecipitation sample by anti-AR antibody indicated VAPA is a candidate protein.
Knockdown of VAPA by shRNA decreased the amount of AR localized to membrane and nuclear fraction and prevented AR trafficking after being exposed to testosterone.
Further studies indicated AR trafficking in Sertoli cells might be mediated by VAPA via association with vesicle transport protein OSBP.
This study can enrich the mechanism of the androgen actions and will be helpful for further clarifying the nonclassical signaling pathway of androgens in Sertoli cells.
American Psychological Association (APA)
Deng, Qiong& Zhang, Jianwen& Wang, Zhu& Zhang, Shengping& Zhi, Fan& Liang, Hui. 2018. Vesicle-Associated Membrane Protein-Associated Protein A Is Involved in Androgen Receptor Trafficking in Mouse Sertoli Cells. International Journal of Endocrinology،Vol. 2018, no. 2018, pp.1-6.
https://search.emarefa.net/detail/BIM-1171407
Modern Language Association (MLA)
Deng, Qiong…[et al.]. Vesicle-Associated Membrane Protein-Associated Protein A Is Involved in Androgen Receptor Trafficking in Mouse Sertoli Cells. International Journal of Endocrinology No. 2018 (2018), pp.1-6.
https://search.emarefa.net/detail/BIM-1171407
American Medical Association (AMA)
Deng, Qiong& Zhang, Jianwen& Wang, Zhu& Zhang, Shengping& Zhi, Fan& Liang, Hui. Vesicle-Associated Membrane Protein-Associated Protein A Is Involved in Androgen Receptor Trafficking in Mouse Sertoli Cells. International Journal of Endocrinology. 2018. Vol. 2018, no. 2018, pp.1-6.
https://search.emarefa.net/detail/BIM-1171407
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1171407