Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Production by Rhodospirillum rubrum Using a Two-Step Culture Strategy
Joint Authors
Cheng, Yanling
Liu, Jie
Zhao, Youxi
Diao, Mengjie
Wang, Wanqing
Hua, Wei
Wu, Shuang
Chen, Paul
Ruan, Roger
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-08-04
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Polyhydroxyalkanoates (PHAs) are microbially synthesized biopolyesters which have attracted great attentions as a new biological material, potential alternative to traditional fossil fuel-based plastic due to their biodegradability and biocompatibility.
Poly-3-hydroxybutyrate (PHB) and poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) are the most common members of PHAs.
In this study, the nonsulfur and facultatively phototrophic bacterium Rhodospirillum rubrum was cultivated to accumulate PHA by a two-step culture strategy.
Gas chromatography (GC), gas chromatography-mass spectrometry (GC-MS), and nuclear magnetic resonance spectroscopy (NMR) analyses showed that PHAs synthesized from fructose was PHBV, in which the 3HV content was 46.5 mol%, which means the better mechanical property.
The molecular weight, distribution, and thermal features were characterized by gel permeation chromatography (GPC), differential scanning calorimeter (DSC), and thermo gravimetric analysis (TGA), respectively.
The low PDI of 1.08 revealed the narrow and evenly molar mass distribution which shows the stable features.
The high melting temperature and their other physical properties implied their potential applications.
The traditional process of producing PHBV involves related carbon sources such as valeric acid.
However, our study clearly described a new medium formula with fructose and a complete fermentation method to produce PHBV with a high 3HV faction and low molecular distribution.
American Psychological Association (APA)
Liu, Jie& Zhao, Youxi& Diao, Mengjie& Wang, Wanqing& Hua, Wei& Wu, Shuang…[et al.]. 2019. Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Production by Rhodospirillum rubrum Using a Two-Step Culture Strategy. Journal of Chemistry،Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1171854
Modern Language Association (MLA)
Liu, Jie…[et al.]. Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Production by Rhodospirillum rubrum Using a Two-Step Culture Strategy. Journal of Chemistry No. 2019 (2019), pp.1-8.
https://search.emarefa.net/detail/BIM-1171854
American Medical Association (AMA)
Liu, Jie& Zhao, Youxi& Diao, Mengjie& Wang, Wanqing& Hua, Wei& Wu, Shuang…[et al.]. Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Production by Rhodospirillum rubrum Using a Two-Step Culture Strategy. Journal of Chemistry. 2019. Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1171854
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1171854