Caveolin-1 Scaffolding Domain Peptide Regulates Colon Endothelial Cell Survival through JNK Pathway
Joint Authors
Fang, Kai
Kevil, Christopher G.
Source
International Journal of Inflammation
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-03-06
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
It has been reported that pathological angiogenesis contributes to both experimental colitis and inflammatory bowel disease.
Recently, we demonstrated that endothelial caveolin-1 plays a key role in the pathological angiogenesis of dextran sodium sulfate (DSS) colitis.
However, the molecular mechanism of caveolin-1 regulation of endothelial function is unknown.
In this study, we examined how the antennapedia- (AP-) conjugated caveolin-1 scaffolding domain (AP-Cav) modulates vascular endothelial growth factor- (VEGF-) dependent colon endothelial cell angiogenic responses, as seen during colitis.
We used mouse colon endothelial cells and found that AP-Cav significantly inhibited VEGF-mediated bromodeoxyuridine (BrdU) incorporation into colon microvascular endothelial cells.
AP-Cav significantly blunted VEGF-dependent extracellular signal-regulated kinase 1/2 (ERK 1/2) phosphorylation at 10 minutes and 2 hours after stimulation, compared with the AP control peptide.
AP-Cav + VEGF-A treatment also significantly increased c-Jun N-terminal kinase (JNK) phosphorylation at 2 hours.
AP-Cav + VEGF-A treatment significantly downregulated retinoblastoma (Rb) protein levels, upregulated cleaved caspase-3 protein levels at 4 hours, and induced apoptosis.
Thus, our study suggests that disruption of endothelial caveolin-1 function via the AP-Cav diverts VEGF signaling responses away from endothelial cell proliferation and toward apoptosis through the inhibition of mitogen-activated protein (MAP) kinase signaling and the induction of JNK-associated apoptosis.
American Psychological Association (APA)
Fang, Kai& Kevil, Christopher G.. 2020. Caveolin-1 Scaffolding Domain Peptide Regulates Colon Endothelial Cell Survival through JNK Pathway. International Journal of Inflammation،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1171862
Modern Language Association (MLA)
Fang, Kai& Kevil, Christopher G.. Caveolin-1 Scaffolding Domain Peptide Regulates Colon Endothelial Cell Survival through JNK Pathway. International Journal of Inflammation No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1171862
American Medical Association (AMA)
Fang, Kai& Kevil, Christopher G.. Caveolin-1 Scaffolding Domain Peptide Regulates Colon Endothelial Cell Survival through JNK Pathway. International Journal of Inflammation. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1171862
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1171862