In Vitro and In Vivo Antifungal Activity of Clove (Eugenia caryophyllata) and Pepper (Piper nigrum L.) Essential Oils and Functional Extracts Against Fusarium oxysporum and Aspergillus niger in Tomato (Solanum lycopersicum L.)
Joint Authors
Sandoval-Salas, Fabiola
Ayala-Soto, Juan Guillermo
Hernández-Ochoa, León
Muñoz Castellanos, Laila
Amaya Olivas, Nubia
De La O Contreras, Carmen Miriam
Zermeño Ortega, Miriam
Source
International Journal of Microbiology
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-05-01
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
In this study, hydrodistillation was used to obtain essential oils (EOs) from pepper (Piper nigrum L.) and clove (Eugenia caryophyllata) and co-hydrodistillation (addition of fatty acid ethyl esters as extraction co-solvents) was used to obtain functional extracts (FEs).
Antifungal activity of EOs and FEs was evaluated by determination of minimum inhibitory concentration (MIC) against Fusarium oxysporum and Aspergillus niger.
The results showed that pepper (Piper nigrum) and clove (Eugenia caryophyllata) essential oils and their functional extracts are effective in vitro at concentrations from 400 to 500 ppm after 10 days of culturing.
The essential oils and functional extracts were used on tomato fruit samples at three different concentrations: 350, 400, and 450 ppm5.
Clove essential oil reduced the growth of Aspergillus niger from 50% to 70% and Fusarium oxysporum to 40%.
The functional extracts (FEs) of clove and pepper, mixed with ethyl decanoate (FEs-C10), were the best combination for protecting the tomato fruit in vivo against both phytopathogenic fungi.
Gas chromatography-mass spectrometry (GC-MS) was used to identify eugenol as the principal compound in clove oil and limonene, sabinene, and β-caryophyllene in pepper oil.
American Psychological Association (APA)
Muñoz Castellanos, Laila& Amaya Olivas, Nubia& Ayala-Soto, Juan Guillermo& De La O Contreras, Carmen Miriam& Zermeño Ortega, Miriam& Sandoval-Salas, Fabiola…[et al.]. 2020. In Vitro and In Vivo Antifungal Activity of Clove (Eugenia caryophyllata) and Pepper (Piper nigrum L.) Essential Oils and Functional Extracts Against Fusarium oxysporum and Aspergillus niger in Tomato (Solanum lycopersicum L.). International Journal of Microbiology،Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1171987
Modern Language Association (MLA)
Muñoz Castellanos, Laila…[et al.]. In Vitro and In Vivo Antifungal Activity of Clove (Eugenia caryophyllata) and Pepper (Piper nigrum L.) Essential Oils and Functional Extracts Against Fusarium oxysporum and Aspergillus niger in Tomato (Solanum lycopersicum L.). International Journal of Microbiology No. 2020 (2020), pp.1-8.
https://search.emarefa.net/detail/BIM-1171987
American Medical Association (AMA)
Muñoz Castellanos, Laila& Amaya Olivas, Nubia& Ayala-Soto, Juan Guillermo& De La O Contreras, Carmen Miriam& Zermeño Ortega, Miriam& Sandoval-Salas, Fabiola…[et al.]. In Vitro and In Vivo Antifungal Activity of Clove (Eugenia caryophyllata) and Pepper (Piper nigrum L.) Essential Oils and Functional Extracts Against Fusarium oxysporum and Aspergillus niger in Tomato (Solanum lycopersicum L.). International Journal of Microbiology. 2020. Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1171987
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1171987