Reprocessing of Contaminated MGIT 960 Cultures to Improve Availability of Valid Results for Mycobacteria
Joint Authors
Subramanyam, Balaji
Sivaramakrishnan, Gomathi
Sangamithrai, Devi
Ravi, Rajkumar
Thiruvengadam, Kannan
Vijayaragavan, Vaishnavi
Selvaraju, Ayswarya
Tripathy, Srikanth Prasad
Mondal, Rajesh
Source
International Journal of Microbiology
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-3, 3 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-07-18
Country of Publication
Egypt
No. of Pages
3
Main Subjects
Abstract EN
Optimal recovery of mycobacteria from the contaminated liquid culture is a challenge.
While alternative methods have been suggested to reduce the rate of contamination in the BACTEC MGIT 960 system, reprocessing the contaminated liquid culture improves recovery of Mycobacterium tuberculosis.
Among 793 MGIT cultures raised from as many sputum specimens after primary decontamination by the standard NaLC-NaOH method, valid results were available for 687 (86.6%) as 106 (13.4%) were contaminated.
Reprocessing and reculturing of the contaminated cultures increased valid results to 739 (93.2%) and reduced the contamination rate to 6.8%.
Both values were statistically significant.
Recovery of the Mycobacterium tuberculosis complex increased from 45.6% to 48.4%.
Valid negative results were available for an additional 3.4%.
The method may be adopted to reduce the rate of contamination and to improve the valid culture results for mycobacteria.
American Psychological Association (APA)
Subramanyam, Balaji& Sivaramakrishnan, Gomathi& Sangamithrai, Devi& Ravi, Rajkumar& Thiruvengadam, Kannan& Vijayaragavan, Vaishnavi…[et al.]. 2020. Reprocessing of Contaminated MGIT 960 Cultures to Improve Availability of Valid Results for Mycobacteria. International Journal of Microbiology،Vol. 2020, no. 2020, pp.1-3.
https://search.emarefa.net/detail/BIM-1171994
Modern Language Association (MLA)
Subramanyam, Balaji…[et al.]. Reprocessing of Contaminated MGIT 960 Cultures to Improve Availability of Valid Results for Mycobacteria. International Journal of Microbiology No. 2020 (2020), pp.1-3.
https://search.emarefa.net/detail/BIM-1171994
American Medical Association (AMA)
Subramanyam, Balaji& Sivaramakrishnan, Gomathi& Sangamithrai, Devi& Ravi, Rajkumar& Thiruvengadam, Kannan& Vijayaragavan, Vaishnavi…[et al.]. Reprocessing of Contaminated MGIT 960 Cultures to Improve Availability of Valid Results for Mycobacteria. International Journal of Microbiology. 2020. Vol. 2020, no. 2020, pp.1-3.
https://search.emarefa.net/detail/BIM-1171994
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1171994