Molecular Cloning and Expression Analysis of CuZn SOD Gene from Gynura bicolor DC.
Joint Authors
Xu, Xin
Yu, Zhi-fang
Kong, Jing
Yi, Mei-jun
Huan, Chen
Jiang, Li
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-04-18
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Superoxide dismutase is an important antioxidant enzyme extensively existing in eukaryote, which scavenges reactive oxygen species (ROS) and plays an essential role in stress tolerance of higher plants.
A full-length cDNA encoding Cu/Zn SOD was cloned from leaves of Gynura bicolor DC.
by rapid amplification of cDNA ends (RACE).
The full-length cDNA of Cu/Zn SOD is 924 bp and has a 681 bp open reading frame encoding 227 amino acids.
Bioinformatics analysis revealed that belonged to the plant SOD super family.
Cu/Zn SODs of the Helianthus annuus, Mikania micrantha, and Solidago canadensis var.
scabra all have 86% similarity to the G.
bicolor Cu/Zn SOD.
Analysis of the expression of Cu/Zn SOD under different treatments revealed that Cu/Zn SOD was a stress-responsive gene, especially to 1-MCP.
It indicates that the Cu/Zn SOD gene would be an important gene in the resistance to stresses and will be helpful in providing evidence for future research on underlying molecular mechanism and choosing proper postharvest treatments for G.
bicolor.
American Psychological Association (APA)
Xu, Xin& Yu, Zhi-fang& Kong, Jing& Yi, Mei-jun& Huan, Chen& Jiang, Li. 2017. Molecular Cloning and Expression Analysis of CuZn SOD Gene from Gynura bicolor DC.. Journal of Chemistry،Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1172101
Modern Language Association (MLA)
Xu, Xin…[et al.]. Molecular Cloning and Expression Analysis of CuZn SOD Gene from Gynura bicolor DC.. Journal of Chemistry No. 2017 (2017), pp.1-8.
https://search.emarefa.net/detail/BIM-1172101
American Medical Association (AMA)
Xu, Xin& Yu, Zhi-fang& Kong, Jing& Yi, Mei-jun& Huan, Chen& Jiang, Li. Molecular Cloning and Expression Analysis of CuZn SOD Gene from Gynura bicolor DC.. Journal of Chemistry. 2017. Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1172101
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1172101