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Differentiation of Candida albicans Species Complex by Tobacco Agar Obtained from Different Cigarette Brands Available in Colombia
Joint Authors
Morales-López, Soraya E.
Yepes, Jayr
Elles, Danna C.
Macías, Lisahidy
Cañate, Abid
Robles Camargo, Jorge
Source
International Journal of Microbiology
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-03-21
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
Introduction.
The Candida albicans complex is formed by Candida albicans, Candida dubliniensis, and a biovar of C.
albicans named Candida africana.
These yeasts are recognized as globally distributed clinical pathogens and share most phenotypic characteristics, which makes their discrimination by conventional methods difficult.
Aim.
To evaluate the efficacy of different brands of cigarettes in the preparation of tobacco agar, for the differentiation of these related yeasts.
Methodology.
Tobacco agar was prepared using six brands and four varieties of cigarettes, and 125 clinical isolates previously identified by PCR and Maldi-Tof were used.
To determine whether the results of the microbiological tests were associated with similarities in the chemical components of cigarettes, thin-layer chromatography was performed.
Results.
Candida dubliniensis colonies presented hue differences according to the incubation temperature and the brand or variety of cigarette used, except in the tobacco agar produced with Marlboro Xpress cigarette, where its differentiation was not possible.
The chromatograms showed few differences among apolar and medium polarity extract components.
Conclusions.
Tobacco agar is a low-cost tool used for the differentiation of Candida dubliniensis; however, incubation temperature and cigarette brand affect the performance of the media.
No relationship was found between the microbiological results and the chemical similarity of the extracts of the cigarettes by chromatography.
American Psychological Association (APA)
Morales-López, Soraya E.& Yepes, Jayr& Elles, Danna C.& Macías, Lisahidy& Cañate, Abid& Robles Camargo, Jorge. 2020. Differentiation of Candida albicans Species Complex by Tobacco Agar Obtained from Different Cigarette Brands Available in Colombia. International Journal of Microbiology،Vol. 2020, no. 2020, pp.1-5.
https://search.emarefa.net/detail/BIM-1172166
Modern Language Association (MLA)
Morales-López, Soraya E.…[et al.]. Differentiation of Candida albicans Species Complex by Tobacco Agar Obtained from Different Cigarette Brands Available in Colombia. International Journal of Microbiology No. 2020 (2020), pp.1-5.
https://search.emarefa.net/detail/BIM-1172166
American Medical Association (AMA)
Morales-López, Soraya E.& Yepes, Jayr& Elles, Danna C.& Macías, Lisahidy& Cañate, Abid& Robles Camargo, Jorge. Differentiation of Candida albicans Species Complex by Tobacco Agar Obtained from Different Cigarette Brands Available in Colombia. International Journal of Microbiology. 2020. Vol. 2020, no. 2020, pp.1-5.
https://search.emarefa.net/detail/BIM-1172166
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1172166