Antibacterial and Anticancer Activity and Untargeted Secondary Metabolite Profiling of Crude Bacterial Endophyte Extracts from Crinum macowanii Baker Leaves
Joint Authors
Green, Ezekiel
Sebola, Tendani E.
Uche-Okereafor, Nkemdinma C.
Mekuto, Lukhanyo
Makatini, Maya Mellisa
Mavumengwana, Vuyo
Source
International Journal of Microbiology
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-12-10
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
This study isolated and identified endophytic bacteria from the leaves of Crinum macowanii and investigated the potential of the bacterial endophyte extracts as antibacterial and anticancer agents and their subsequent secondary metabolites.
Ethyl acetate extracts from the endophytes and the leaves (methanol: dichloromethane (1 : 1)) were used for antibacterial activity against selected pathogenic bacterial strains by using the broth microdilution method.
The anticancer activity against the U87MG glioblastoma and A549 lung carcinoma cells was determined by the MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxy-phenyl)-2-(4-sulfophenyl)-2H-tetrazolium) assay.
Bacterial endophytes that were successfully isolated from C.
macowanii leaves include Raoultella ornithinolytica, Acinetobacter guillouiae, Pseudomonas sp., Pseudomonas palleroniana, Pseudomonas putida, Bacillus safensis, Enterobacter asburiae, Pseudomonas cichorii, and Arthrobacter pascens.
Pseudomonas cichorii exhibited broad antibacterial activity against both Gram-negative and Gram-positive pathogenic bacteria while Arthrobacter pascens displayed the least MIC of 0.0625 mg/mL.
Bacillus safensis crude extracts were the only sample that showed notable cell reduction of 50% against A549 lung carcinoma cells at a concentration of 100 μg/mL.
Metabolite profiling of Bacillus safensis, Pseudomonas cichorii, and Arthrobacter pascens crude extracts revealed the presence of known antibacterial and/or anticancer agents such as lycorine (1), angustine (2), crinamidine (3), vasicinol (4), and powelline.
It can be concluded that the crude bacterial endophyte extracts obtained from C.
macowanii leaves can biosynthesize bioactive compounds and can be bioprospected for medical application into antibacterial and anticancer agents.
American Psychological Association (APA)
Sebola, Tendani E.& Uche-Okereafor, Nkemdinma C.& Mekuto, Lukhanyo& Makatini, Maya Mellisa& Green, Ezekiel& Mavumengwana, Vuyo. 2020. Antibacterial and Anticancer Activity and Untargeted Secondary Metabolite Profiling of Crude Bacterial Endophyte Extracts from Crinum macowanii Baker Leaves. International Journal of Microbiology،Vol. 2020, no. 2020, pp.1-15.
https://search.emarefa.net/detail/BIM-1172390
Modern Language Association (MLA)
Sebola, Tendani E.…[et al.]. Antibacterial and Anticancer Activity and Untargeted Secondary Metabolite Profiling of Crude Bacterial Endophyte Extracts from Crinum macowanii Baker Leaves. International Journal of Microbiology No. 2020 (2020), pp.1-15.
https://search.emarefa.net/detail/BIM-1172390
American Medical Association (AMA)
Sebola, Tendani E.& Uche-Okereafor, Nkemdinma C.& Mekuto, Lukhanyo& Makatini, Maya Mellisa& Green, Ezekiel& Mavumengwana, Vuyo. Antibacterial and Anticancer Activity and Untargeted Secondary Metabolite Profiling of Crude Bacterial Endophyte Extracts from Crinum macowanii Baker Leaves. International Journal of Microbiology. 2020. Vol. 2020, no. 2020, pp.1-15.
https://search.emarefa.net/detail/BIM-1172390
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1172390