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Detection of bla NDM-1 Encoding Imepenemase among the Imipenem-Resistant Gram-Negative Bacilli Isolated from Various Clinical Samples at a Tertiary Care Hospital of Eastern Nepal: A Descriptive Cross-Sectional Study
Joint Authors
Gautam, S.
Bhattarai, N. R.
Rai, K.
Poudyal, A.
Khanal, B.
Source
International Journal of Microbiology
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-07-27
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
Background.
Carbapenem resistance among Gram-negative isolates caused by the production of the metallo-β-lactamase (MBL) enzyme is being increasingly reported worldwide.
One of the newly emerged metallo-β-lactamases is New Delhi metallo-β-lactamase.
Data regarding its occurrence in hospital setting and percentage prevalence among different Gram-negative bacterial isolates are lacking in our part.
This study has been undertaken for determining the presence of the bla NDM-1 gene among the clinical isolates of imipenem-resistant Gram-negative bacteria in a tertiary care center in Dharan, Nepal.
Methods.
A total of 75 imipenem-resistant Gram-negative isolates were studied.
These were screened for metallo-β-lactamase (MBL) production by phenotypic assays such as double-disc synergy test (DDST) and combined disc diffusion test (CDDT).
PCR was performed for the molecular detection of gene NDM-1.
Ten-disc method was performed to detect the presence of ESBL, AmpC, carbapenamase, and K1 β-lactamase production.
Results.
Using the molecular method, bla NDM-1 was detected in 36% of the isolates.
Phenotypically, double-disc synergy test (DDST) and combined disc diffusion test (CDST) detected MBL production in 38.7% and 37.3% of the isolates, respectively.
Ten-disc method detected ESBL in 26.6% of the isolates, but none of the isolates was found to be AmpC, carbapenamase, and K1 β-lactamase producers.
Conclusion.
A high percentage of the NDM-1 producer was noted among imipenem-resistant GNB.
Apart from performing only antimicrobial sensitivity test, phenotypic and molecular screening should be employed to find out the actual number of metallo-β-lactamase producers and the existence of the resistance gene.
American Psychological Association (APA)
Gautam, S.& Bhattarai, N. R.& Rai, K.& Poudyal, A.& Khanal, B.. 2020. Detection of bla NDM-1 Encoding Imepenemase among the Imipenem-Resistant Gram-Negative Bacilli Isolated from Various Clinical Samples at a Tertiary Care Hospital of Eastern Nepal: A Descriptive Cross-Sectional Study. International Journal of Microbiology،Vol. 2020, no. 2020, pp.1-5.
https://search.emarefa.net/detail/BIM-1172446
Modern Language Association (MLA)
Gautam, S.…[et al.]. Detection of bla NDM-1 Encoding Imepenemase among the Imipenem-Resistant Gram-Negative Bacilli Isolated from Various Clinical Samples at a Tertiary Care Hospital of Eastern Nepal: A Descriptive Cross-Sectional Study. International Journal of Microbiology No. 2020 (2020), pp.1-5.
https://search.emarefa.net/detail/BIM-1172446
American Medical Association (AMA)
Gautam, S.& Bhattarai, N. R.& Rai, K.& Poudyal, A.& Khanal, B.. Detection of bla NDM-1 Encoding Imepenemase among the Imipenem-Resistant Gram-Negative Bacilli Isolated from Various Clinical Samples at a Tertiary Care Hospital of Eastern Nepal: A Descriptive Cross-Sectional Study. International Journal of Microbiology. 2020. Vol. 2020, no. 2020, pp.1-5.
https://search.emarefa.net/detail/BIM-1172446
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1172446