Potential Pathways Involved in Elaidic Acid Induced Atherosclerosis in Human Umbilical Vein Endothelial Cells
Joint Authors
Li, Hong-Yan
Deng, Ze-Yuan
Li, Si-guang
Yu, Huahong
Li, Xiangmei
Liang, Zhongshang
Qiu, Bin
Luo, Ting
Li, Jing
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-06-08
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Researches have demonstrated that trans-fatty acids are related to the progression of atherosclerosis, but the underlying mechanism is not clear till now.
In the presented study, two-dimensional electrophoresis based proteomics was used to discover the role of elaidic acid in atherosclerosis.
In human umbilical vein endothelial cells (HUVEC), twenty-two and twenty-three differentially expressed proteins were identified in low (50 μmol/L) and high (400 μmol/L) concentration elaidic acid simulated groups, respectively, comparing with the control group.
The expressions of some selected proteins (PSME3, XRCC5, GSTP1, and GSTO1) were validated by qRT-PCR analysis.
Western blotting analysis further confirmed that elaidic acid downregulated the expression of PSME3 and XRCC5.
Moreover, P53, the downstream protein of PSME3, was further investigated.
Results demonstrated that a variety of proteins, many of which were related to oxidative stress, apoptosis, and DNA damage, were involved in the elaidic acid induced atherosclerosis.
Furthermore, P53 was demonstrated to regulate the atherosclerosis through cell cycle arrest and apoptosis pathway.
American Psychological Association (APA)
Yu, Huahong& Li, Xiangmei& Liang, Zhongshang& Qiu, Bin& Li, Si-guang& Luo, Ting…[et al.]. 2017. Potential Pathways Involved in Elaidic Acid Induced Atherosclerosis in Human Umbilical Vein Endothelial Cells. Journal of Chemistry،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1172525
Modern Language Association (MLA)
Yu, Huahong…[et al.]. Potential Pathways Involved in Elaidic Acid Induced Atherosclerosis in Human Umbilical Vein Endothelial Cells. Journal of Chemistry No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1172525
American Medical Association (AMA)
Yu, Huahong& Li, Xiangmei& Liang, Zhongshang& Qiu, Bin& Li, Si-guang& Luo, Ting…[et al.]. Potential Pathways Involved in Elaidic Acid Induced Atherosclerosis in Human Umbilical Vein Endothelial Cells. Journal of Chemistry. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1172525
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1172525